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AB69565

Anti-HIPK4 antibody

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(1 Publication)

Rabbit Polyclonal HIPK4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HIPK4.

View Alternative Names

Homeodomain-interacting protein kinase 4, HIPK4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIPK4 antibody (AB69565)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIPK4 antibody (AB69565)

Immunofluorescence analysis of A549 cells, using 1 : 500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPK4 antibody (AB69565)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPK4 antibody (AB69565)

Immunohistochemistry analysis of paraffin-embedded human testis tissue using 1 : 50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.

Western blot - Anti-HIPK4 antibody (AB69565)
  • WB

Unknown

Western blot - Anti-HIPK4 antibody (AB69565)

All lanes:

Western blot - Anti-HIPK4 antibody (ab69565) at 1/500 dilution

Lane 1:

extracts from HUVEC cells at 30 µg

Lane 2:

extracts from HUVEC cells at 30 µg with immunizing peptide

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human HIPK4. The exact immunogen used to generate this antibody is proprietary information.

Q8NE63

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIPK4 known as Homeodomain Interacting Protein Kinase 4 acts as a serine/threonine-protein kinase. It weighs around 69 kDa. This protein primarily localizes in the nucleus and occasionally the cytoplasm across various tissues. HIPK4 participates in phosphorylation processes modifying the activity of multiple substrates. Researchers often study it for its potential interactions with different transcription factors and regulators.
Biological function summary

This protein plays an important role in regulating cell growth and differentiation. HIPK4 does not function as part of a complex but acts independently to impact these cellular processes. It can influence the expression of genes involved in apoptosis and cell cycle regulation indicating its significant influence on cellular homeostasis.

Pathways

Researchers recognize HIPK4's involvement in the Wnt signaling and JNK pathways. The protein interacts with other kinases such as HIPK2 which also play roles in these pathways. These interactions suggest that HIPK4 might have diverse biological effects including those on development and cancer evolution.

Scientists have linked HIPK4 to cancer and neurodegenerative diseases. It interacts with proteins like p53 which are important in tumor suppression. HIPK4's alteration may contribute to the development of cancer by disturbing the balance of cell proliferation and death. Additionally its dysregulation could potentially connect to neuronal cell death implicating a role in neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
See full target information HIPK4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112633 PubMed37314927

2023

Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Blumrich,Jessica C Nicholson-Fish,Marie Pronot,Elizabeth C Davenport,Dominic Kurian,Adam Cole,Karen J Smillie,Michael A Cousin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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