Rabbit Polyclonal HIPK4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HIPK4.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human HIPK4. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NE63
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
Alternative names
Homeodomain-interacting protein kinase 4, HIPK4
Recommended products
Rabbit Polyclonal HIPK4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HIPK4.
Alternative names
Homeodomain-interacting protein kinase 4, HIPK4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human HIPK4. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NE63
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIPK4 known as Homeodomain Interacting Protein Kinase 4 acts as a serine/threonine-protein kinase. It weighs around 69 kDa. This protein primarily localizes in the nucleus and occasionally the cytoplasm across various tissues. HIPK4 participates in phosphorylation processes modifying the activity of multiple substrates. Researchers often study it for its potential interactions with different transcription factors and regulators.
- Biological function summary
This protein plays an important role in regulating cell growth and differentiation. HIPK4 does not function as part of a complex but acts independently to impact these cellular processes. It can influence the expression of genes involved in apoptosis and cell cycle regulation indicating its significant influence on cellular homeostasis.
- Pathways
Researchers recognize HIPK4's involvement in the Wnt signaling and JNK pathways. The protein interacts with other kinases such as HIPK2 which also play roles in these pathways. These interactions suggest that HIPK4 might have diverse biological effects including those on development and cancer evolution.
- Associated diseases and disorders
Scientists have linked HIPK4 to cancer and neurodegenerative diseases. It interacts with proteins like p53 which are important in tumor suppression. HIPK4's alteration may contribute to the development of cancer by disturbing the balance of cell proliferation and death. Additionally its dysregulation could potentially connect to neuronal cell death implicating a role in neurodegenerative conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-HIPK4 antibody (ab69565)
All lanes: Western blot - Anti-HIPK4 antibody (ab69565) at 1/500 dilution
All lanes: extracts from HUVEC cells at 30 µg
Predicted band size: 69 kDa
Observed band size: 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPK4 antibody (ab69565)
Immunohistochemistry analysis of paraffin-embedded
human testis tissue using 1:50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-HIPK4 antibody (ab69565)
Immunofluorescence analysis of A549 cells, using 1:500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.
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