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AB154601

Anti-HIPPI/IFT57 antibody

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(1 Publication)

Rabbit Polyclonal HIPPI/IFT57 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFT57 aa 200 to C-terminus.

View Alternative Names

DERP8, ESRRBL1, HIPPI, IFT57, Intraflagellar transport protein 57 homolog, Dermal papilla-derived protein 8, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein, MHS4R2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (AB154601)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (AB154601)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (AB154601)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (AB154601)

Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.

Western blot - Anti-HIPPI/IFT57 antibody (AB154601)
  • WB

Unknown

Western blot - Anti-HIPPI/IFT57 antibody (AB154601)

10% SDS PAGE

All lanes:

Western blot - Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IFT57 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NWB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIPPI also known as IFT57 (Intraflagellar Transport Protein 57) is a protein with a mass of approximately 42 kDa. It plays a role in intracellular transport particularly linked to the movement of materials along cilia and flagella. Expression of HIPPI is observed in various tissues notably in neural tissues where it contributes to cellular organization and function. Its involvement in microtubule-based processes highlights its significance in cellular transport mechanisms.
Biological function summary

The protein operates as part of the intraflagellar transport (IFT) complex B functioning in the assembly and maintenance of cilia. HIPPI works closely with other IFT proteins like IFT88 and IFT20 coordinating the movement of protein complexes necessary for ciliary structure and biogenesis. The formation and function of cilia are critical for cellular signaling and movement reflecting HIPPI’s role in supporting these processes.

Pathways

HIPPI integrates into the Hedgehog signaling and Wnt pathways essential for development and cellular differentiation. These pathways involve key proteins like SMO within the Hedgehog pathway and β-catenin in the Wnt pathway linking HIPPI's role in ciliogenesis to broader signaling networks that dictate cell proliferation and patterning. These interactions highlight HIPPI’s participation in major biological pathways that influence cellular communication and development.

HIPPI shows a connection to neurodegenerative conditions and ciliopathies. Evidence links HIPPI to disorders such as Alzheimer’s disease where it interacts with proteins like presenilin influencing neurodegeneration processes. Additionally HIPPI's role in ciliopathies connects it to diseases linked to defects in ciliary function. These associations illustrate HIPPI's involvement in conditions where impaired cellular transport and signaling lead to pathological outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
See full target information IFT57
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 15:506-22 PubMed26598648

2015

Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1.

Applications

Unspecified application

Species

Unspecified reactive species

Noga Gershoni-Emek,Arnon Mazza,Michael Chein,Tal Gradus-Pery,Xin Xiang,Ka Wan Li,Roded Sharan,Eran Perlson
View all publications
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Product promise

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