Rabbit Polyclonal HIPPI/IFT57 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
DERP8, ESRRBL1, HIPPI, IFT57, DERP8, ESRRBL1, HIPPI, Intraflagellar transport protein 57 homolog, Dermal papilla-derived protein 8, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein, MHS4R2
Rabbit Polyclonal HIPPI/IFT57 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Keep as concentrated solution.
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This supplementary information is collated from multiple sources and compiled automatically.
HIPPI also known as IFT57 (Intraflagellar Transport Protein 57) is a protein with a mass of approximately 42 kDa. It plays a role in intracellular transport particularly linked to the movement of materials along cilia and flagella. Expression of HIPPI is observed in various tissues notably in neural tissues where it contributes to cellular organization and function. Its involvement in microtubule-based processes highlights its significance in cellular transport mechanisms.
The protein operates as part of the intraflagellar transport (IFT) complex B functioning in the assembly and maintenance of cilia. HIPPI works closely with other IFT proteins like IFT88 and IFT20 coordinating the movement of protein complexes necessary for ciliary structure and biogenesis. The formation and function of cilia are critical for cellular signaling and movement reflecting HIPPI’s role in supporting these processes.
HIPPI integrates into the Hedgehog signaling and Wnt pathways essential for development and cellular differentiation. These pathways involve key proteins like SMO within the Hedgehog pathway and β-catenin in the Wnt pathway linking HIPPI's role in ciliogenesis to broader signaling networks that dictate cell proliferation and patterning. These interactions highlight HIPPI’s participation in major biological pathways that influence cellular communication and development.
HIPPI shows a connection to neurodegenerative conditions and ciliopathies. Evidence links HIPPI to disorders such as Alzheimer’s disease where it interacts with proteins like presenilin influencing neurodegeneration processes. Additionally HIPPI's role in ciliopathies connects it to diseases linked to defects in ciliary function. These associations illustrate HIPPI's involvement in conditions where impaired cellular transport and signaling lead to pathological outcomes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
10% SDS PAGE
All lanes: Western blot - Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 49 kDa
Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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