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Rabbit Polyclonal HIPPI/IFT57 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus.

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Images

Western blot - Anti-HIPPI/IFT57 antibody (AB154601), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (AB154601), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (AB154601), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NWB7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Target data

Function

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Alternative names

Recommended products

Rabbit Polyclonal HIPPI/IFT57 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Intraflagellar transport protein 57 homolog aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NWB7
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Keep as concentrated solution.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HIPPI also known as IFT57 (Intraflagellar Transport Protein 57) is a protein with a mass of approximately 42 kDa. It plays a role in intracellular transport particularly linked to the movement of materials along cilia and flagella. Expression of HIPPI is observed in various tissues notably in neural tissues where it contributes to cellular organization and function. Its involvement in microtubule-based processes highlights its significance in cellular transport mechanisms.

Biological function summary

The protein operates as part of the intraflagellar transport (IFT) complex B functioning in the assembly and maintenance of cilia. HIPPI works closely with other IFT proteins like IFT88 and IFT20 coordinating the movement of protein complexes necessary for ciliary structure and biogenesis. The formation and function of cilia are critical for cellular signaling and movement reflecting HIPPI’s role in supporting these processes.

Pathways

HIPPI integrates into the Hedgehog signaling and Wnt pathways essential for development and cellular differentiation. These pathways involve key proteins like SMO within the Hedgehog pathway and β-catenin in the Wnt pathway linking HIPPI's role in ciliogenesis to broader signaling networks that dictate cell proliferation and patterning. These interactions highlight HIPPI’s participation in major biological pathways that influence cellular communication and development.

Associated diseases and disorders

HIPPI shows a connection to neurodegenerative conditions and ciliopathies. Evidence links HIPPI to disorders such as Alzheimer’s disease where it interacts with proteins like presenilin influencing neurodegeneration processes. Additionally HIPPI's role in ciliopathies connects it to diseases linked to defects in ciliary function. These associations illustrate HIPPI's involvement in conditions where impaired cellular transport and signaling lead to pathological outcomes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-HIPPI/IFT57 antibody (ab154601), expandable thumbnail

    Western blot - Anti-HIPPI/IFT57 antibody (ab154601)

    10% SDS PAGE

    All lanes: Western blot - Anti-HIPPI/IFT57 antibody (ab154601) at 1/1000 dilution

    All lanes: Raji whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (ab154601), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPPI/IFT57 antibody (ab154601)

    Immunohistochemical analysis of paraffin-embedded H661 xenograft labeling HIPPI/IFT57 with ab154601 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab154601), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab154601)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling HIPPI/IFT57 with ab154601 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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