Anti-HIRA/HIR antibody [EPR25299-11] is a Rabbit Monoclonal antibody that is used in HIRA/HIR IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples.
HIRA (Histone Cell Cycle Regulator) is a histone chaperone that facilitates replication-independent chromatin assembly by placing the variant histone H3.3 into nucleosomes. It plays a crucial role in the formation of senescence-associated heterochromatin foci (SAHF), which are involved in the irreversible cell cycle changes in senescent cells. Additionally, HIRA is essential for the periodic repression of histone gene transcription during the cell cycle
Images
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401367.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)")
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401366.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)")
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401365.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)")
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401364.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Expected | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Alternative names
DGCR1, HIR, TUPLE1, HIRA, Protein HIRA, TUP1-like enhancer of split protein 1
Recommended products
Anti-HIRA/HIR antibody [EPR25299-11] is a Rabbit Monoclonal antibody that is used in HIRA/HIR IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples.
HIRA (Histone Cell Cycle Regulator) is a histone chaperone that facilitates replication-independent chromatin assembly by placing the variant histone H3.3 into nucleosomes. It plays a crucial role in the formation of senescence-associated heterochromatin foci (SAHF), which are involved in the irreversible cell cycle changes in senescent cells. Additionally, HIRA is essential for the periodic repression of histone gene transcription during the cell cycle
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25299-11
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIRA also known as HIR or HIR/HIRA is a histone chaperone protein that plays an essential role in chromatin remodeling and nucleosome assembly. This protein has a mass of approximately 121 kDa. It is expressed in various tissues with notable presence in the liver heart and skeletal muscle. The HIRA protein interacts with other proteins to regulate the deposition of histone H3.3 which is important for processes like DNA replication and repair.
- Biological function summary
HIRA participates in the formation of a complex known as the HIRA-ASF1A-CABIN1 protein complex. This complex operates in chromatin assembly and modulates the incorporation of histone variant H3.3 affecting gene silencing and transcription regulation. Interacting with histone proteins HIRA serves an important function in genome stability and epigenetic mark establishment.
- Pathways
HIRA influences the histone replacement pathway significantly impacting gene expression and chromatin organization. HIRA works closely with ASF1A and CABIN1 proteins within this pathway. Additionally in the senescence-associated secretory phenotype pathway HIRA contributes to cellular aging processes by regulating transcriptional outcomes. The modulation of these pathways by HIRA exemplifies its key role in maintaining cellular functions.
- Associated diseases and disorders
Defects in HIRA associate with hypertrophic cardiomyopathy and some cancer types such as breast cancer. HIRA alterations can disrupt chromatin dynamics potentially linking it with poor prognostic factors in these diseases. In particular the HIRA gene can interact with other genes involved in cardiomyopathies like MYH7 affecting heart muscle function. Through its role in chromatin remodeling HIRA connects to various pathologies where gene regulation and DNA damage responses are compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401367.jpg "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)")
Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling HIRA/HIR with ab302928 at 1/100 (4.86 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human skin. The section was incubated with ab302928 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401366.jpg "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)")
Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 (4.86 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat cerebrum. The section was incubated with ab302928 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401365.jpg "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)")
Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling HIRA/HIR with ab302928 at 1/100 (4.86 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse lung. The section was incubated with ab302928 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928), expandable thumbnail](https://content.abcam.com/products/images/hira-hir-antibody-epr25299-11-ab302928--immunohistochemistry-img401364.jpg "Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)")
Immunohistochemistry - Anti-HIRA/HIR antibody [EPR25299-11] (ab302928)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 (4.86 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse cerebrum. The section was incubated with ab302928 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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