Anti-Histidine decarboxylase antibody [EPR26392-79]

4 product images

• 

  Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444)

  Low expression: Neuro-2a.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

  Lane 1: Human hypothalamus tissue lysate at 80 µg with 5% NFDM/TBST

  Lane 2: Mouse hypothalamus tissue lysate at 80 µg with 5% NFDM/TBST

  Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 74 kDa, 36 kDa

  Exposure time: 81s

• 

  Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444)

  In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

  All lanes: Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

  Lane 1: RBL-2H3 (rat Peripheral blood basophil) whole cell lysate at 80 µg with 5% NFDM/TBST

  Lane 2: S16 (rat Sciatic nerve epithelial-like) whole cell lysate at 80 µg with 5% NFDM/TBST

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 74 kDa, 15 kDa

  Exposure time: 6s

• 

  Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444)

  Low expression: huh7.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 9525922).

  In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

  All lanes: Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

  Lane 1: KU812 (human Peripheral blood basophil) whole cell lysate at 80 µg with 5% NFDM/TBST

  Lane 2: SR (human pleural effusion lymphoblast) whole cell lysate at 80 µg with 5% NFDM/TBST

  Lane 3: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 80 µg with 5% NFDM/TBST

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 53 kDa, 74 kDa, 15 kDa

  Exposure time: 81s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RBL-2H3 (rat Peripheral blood basophil) cells labelling Histidine decarboxylase with ab315444 at 1/50 (10.18 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in RBL-2H3 cell line.
  
  Negative control: S16 .
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.