Anti-Histidine decarboxylase antibody [EPR26392-79]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RBL-2H3 (rat Peripheral blood basophil) cells labelling Histidine decarboxylase with ab315444 at 1/50 (10.18 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in RBL-2H3 cell line.
Negative control : S16 .
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : huh7.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9525922).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

KU812 (human Peripheral blood basophil) whole cell lysate at 80 µg

Lane 2:

SR (human pleural effusion lymphoblast) whole cell lysate at 80 µg

Lane 3:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 53 kDa,74 kDa,15 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 80 µg

Lane 2:

Mouse hypothalamus tissue lysate at 80 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (AB315444)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Histidine decarboxylase antibody [EPR26392-79] (ab315444) at 1/1000 dilution

Lane 1:

RBL-2H3 (rat Peripheral blood basophil) whole cell lysate at 80 µg

Lane 2:

S16 (rat Sciatic nerve epithelial-like) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa,15 kDa

false

Exposure time: 6s