Mouse Monoclonal H1 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human H1-0.
Images
![Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--flow-cytometry-img169805.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)")
![Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunocytochemistry-img174068.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60810.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human H1-0. Database link P07305
Reactivity data
Select an application| IHC-P | ICC | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Alternative names
H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)
Recommended products
Mouse Monoclonal H1 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human H1-0.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human H1-0. Database link P07305
- Carrier free
- Yes
- Clone number
- HH1/957
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
ab216608 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--flow-cytometry-img169805.jpg "Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)")
Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)
Flow Cytometry analysis of HeLa cells labeling Histone H1 with ab216609 at 2ug/million cells followed by goat anti-mouse IgG (Blue); Isotype Control (Red).
![Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunocytochemistry-img174068.jpg "Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)")
Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)
Immunocytochemistry analysis of HeLa cells labeling Histone H1 with ab216609 at 2ug/mL Green. Phalloidin is used to label the membranes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60810.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)
Formalin-fixed, paraffin-embedded human testicular carcinoma tissue stained for Histone H1 using ab216609 at 1 μg/ml in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-hh1-957-bsa-and-azide-free-ab216609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Histone H1 using ab216609 at 1 μg/ml in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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