AB216609

Anti-Histone H1 antibody [HH1/957] - BSA and Azide free

Name: Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (ab216609) | Abcam
Brand: Abcam Limited
SKU: AB216609

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(1 Publication)

Mouse Monoclonal H1 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human H1-0.

View Alternative Names

H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)

4 Images

Supplier Data

Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Immunocytochemistry analysis of HeLa cells labeling Histone H1 with ab216609 at 2ug/mL Green. Phalloidin is used to label the membranes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Formalin-fixed, paraffin-embedded human testicular carcinoma tissue stained for Histone H1 using ab216609 at 1 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Histone H1 using ab216609 at 1 μg/ml in immunohistochemical analysis.

Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Flow Cytometry analysis of HeLa cells labeling Histone H1 with ab216609 at 2ug/million cells followed by goat anti-mouse IgG (Blue); Isotype Control (Red).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HH1/957

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat, Human

Applications

Flow Cyt, IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human H1-0.

P07305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 min at RT).", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 min at RT).", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab216608 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed : 33238161). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed : 33238161). The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division (PubMed : 7374750).

See full target information H1-0

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 8: PubMed38285876

2024

Cellular origin and molecular mechanisms of lung metastases in patients with aggressive hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhi Gulati,Maggie Lutz,Margaret Hanlon,Ashley Cast,Rebekah Karns,James Geller,Alex Bondoc,Gregory Tiao,Lubov Timchenko,Nikolai A Timchenko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H1 antibody [HH1/957] - BSA and Azide free

Immunocytochemistry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Flow Cytometry - Anti-Histone H1 antibody [HH1/957] - BSA and Azide free (AB216609)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise