Mouse Recombinant Monoclonal H1 antibody. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human H1-8.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (AB218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139001.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (AB218417)")
![Flow Cytometry - Anti-Histone H1 antibody [r1415-1] (AB218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--flow-cytometry-img82926.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Histone H1 antibody [r1415-1] (AB218417)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody [r1415-1] (AB218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunocytochemistry-immunofluorescence-img92290.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody [r1415-1] (AB218417)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (AB218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87395.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (AB218417)")
Publications
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- Journal of virology 96:e01011222022HIV-1 Preintegration Complex Preferentially Integrates the Viral DNA into Nucleosomes Containing Trimethylated Histone 3-Lysine 36 Modification and Flanking Linker DNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicklas Sapp,Nathaniel Burge,Khan Cox,Prem Prakash,Muthukumar Balasubramaniam,Santosh Thapa,Devin Christensen,Min Li,Jared Linderberger,Mamuka Kvaratskhelia,Jui Pandhare,Robert Craigie,Michael G Poirier,Chandravanu DashPubMed 36094316
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human H1-8. Database link Q8IZA3
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling (By similarity).
Alternative names
H1FOO, H1OO, OSH1, H1-8, Histone H1.8, Histone H1oo, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1
Recommended products
Mouse Recombinant Monoclonal H1 antibody. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human H1-8.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human H1-8. Database link Q8IZA3
- Clone number
- r1415-1
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139001.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue labeling Histone H1 using ab218417 at 1 μg/ml.
![Flow Cytometry - Anti-Histone H1 antibody [r1415-1] (ab218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--flow-cytometry-img82926.jpg "Flow Cytometry - Anti-Histone H1 antibody [r1415-1] (ab218417)")
Flow Cytometry - Anti-Histone H1 antibody [r1415-1] (ab218417)
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H1 with ab218417 followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody [r1415-1] (ab218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunocytochemistry-immunofluorescence-img92290.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody [r1415-1] (ab218417)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody [r1415-1] (ab218417)
Immunocytochemistry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H1 with ab218417 followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417), expandable thumbnail](https://content.abcam.com/products/images/histone-h1-antibody-r1415-1-ab218417--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87395.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody [r1415-1] (ab218417)
Immunohistochemical analysis of formalin fixed, paraffin embedded rat pancreas tissue labeling Histone H1 using ab218417 at 1 μg/ml.
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