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AB154111

Anti-Histone H1.0 antibody

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(2 Publications)

Rabbit Polyclonal H1.0 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human H1-0 aa 1 to C-terminus.

View Alternative Names

H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)

Immunohistochemical analysis of paraffin embedded SkHep1 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody (AB154111)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody (AB154111)

Immunofluorescence analysis of 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling Histone H1.0 protein at nucleus using ab154111 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red).

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody (AB154111)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody (AB154111)

Immunofluorescence analysis of U2OS cells fixed in 4% paraformaldehyde at RT for 15 min labelling Histone H1.0 protein at nucleus using ab154111 at a 1/1000 dilution (Green). Phalloidin, a cytoskeleton marker stained at a 1/200 dilution (Red).
Scale bar = 10 µM

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)

Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue labeling Histone H1 with ab154111 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)

Immunohistochemical analysis of mouse brain tissue stained for Histone H1.0 protein at nucleus with ab154111 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for Histone H1.0 protein at nucleus with ab154111 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody (AB154111)

Immunohistochemical analysis of paraffin-embedded rat fore brain tissue stained for Histone H1.0 protein at nucleus on rat fore brain with ab154111 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western blot - Anti-Histone H1.0 antibody (AB154111)
  • WB

Supplier Data

Western blot - Anti-Histone H1.0 antibody (AB154111)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Histone H1.0 antibody (ab154111) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 20 kDa

false

Western blot - Anti-Histone H1.0 antibody (AB154111)
  • WB

Supplier Data

Western blot - Anti-Histone H1.0 antibody (AB154111)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Histone H1.0 antibody (ab154111) at 1/10000 dilution

Lane 1:

HepG2 whole cell extracts at 30 µg

Lane 2:

HepG2 nuclear extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 20 kDa

false

Western blot - Anti-Histone H1.0 antibody (AB154111)
  • WB

Supplier Data

Western blot - Anti-Histone H1.0 antibody (AB154111)

Samples were separated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Histone H1.0 antibody (ab154111) at 1/10000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 20 kDa

false

Western blot - Anti-Histone H1.0 antibody (AB154111)
  • WB

Supplier Data

Western blot - Anti-Histone H1.0 antibody (AB154111)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Histone H1.0 antibody (ab154111) at 1/5000 dilution

All lanes:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human H1-0 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
See full target information H1-0
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:106-122.e9 PubMed34875212

2021

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.

Applications

Unspecified application

Species

Unspecified reactive species

Daoud Sheban,Tom Shani,Roey Maor,Alejandro Aguilera-Castrejon,Nofar Mor,Bernardo Oldak,Merav D Shmueli,Avital Eisenberg-Lerner,Jonathan Bayerl,Jakob Hebert,Sergey Viukov,Guoyun Chen,Assaf Kacen,Vladislav Krupalnik,Valeriya Chugaeva,Shadi Tarazi,Alejandra Rodríguez-delaRosa,Mirie Zerbib,Adi Ulman,Solaiman Masarwi,Meital Kupervaser,Yishai Levin,Efrat Shema,Yael David,Noa Novershtern,Jacob H Hanna,Yifat Merbl

Journal of cellular and molecular medicine 23:5895-5906 PubMed31287224

2019

MicroRNA-351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hong Chen,Xiao-Nan Liu,Yan Peng
View all publications
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