AB134914

Anti-Histone H1.0 antibody [EPR6536]

Name: Anti-Histone H1.0 antibody [EPR6536] (ab134914) | Abcam
Brand: Abcam Limited
SKU: AB134914
Price: 210 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal H1.0 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.

Lab

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Lanes 1 - 3 : Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 A431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

false

Unknown

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

BcPC3 cel lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 21 kDa

false

Carrier free

Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6536

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed : 33238161). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed : 33238161). The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division (PubMed : 7374750).

See full target information H1-0

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:441-459 PubMed38765203

2024

Histone H1.0 couples cellular mechanical behaviors to chromatin structure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaishuai Hu,Douglas J Chapski,Natalie D Gehred,Todd H Kimball,Tatiana Gromova,Angelina Flores,Amy C Rowat,Junjie Chen,René R Sevag Packard,Emily Olszewski,Jennifer Davis,Christoph D Rau,Timothy A McKinsey,Manuel Rosa-Garrido,Thomas M Vondriska

Journal of extracellular vesicles 13:e12430 PubMed38602325

2024

Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Seltmann,Britta Hettich,Seraina Abele,Selina Gurri,Valeria Mantella,Jean-Christophe Leroux,Sabine Werner

Nature communications 11:1792 PubMed32286289

2020

Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Morales Torres,Mary Y Wu,Sebastijan Hobor,Elanor N Wainwright,Matthew J Martin,Harshil Patel,William Grey,Eva Grönroos,Steven Howell,Joana Carvalho,Ambrosius P Snijders,Michael Bustin,Dominique Bonnet,Paul D Smith,Charles Swanton,Michael Howell,Paola Scaffidi

EMBO molecular medicine 12:e11466 PubMed32150356

2020

A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Cangkrama,Mateusz Wietecha,Nicolas Mathis,Rin Okumura,Luca Ferrarese,Dunja Al-Nuaimi,Maria Antsiferova,Reinhard Dummer,Metello Innocenti,Sabine Werner

Frontiers in immunology 10:2176 PubMed31572386

2019

Aggregated NETs Sequester and Detoxify Extracellular Histones.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Knopf,Moritz Leppkes,Georg Schett,Martin Herrmann,Luis E Muñoz

Journal of clinical biochemistry and nutrition 64:36-44 PubMed30705510

2019

Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Fukui,Shunsuke Okihiro,Yuuka Ohfuchi,Minae Hashimoto,Yugo Kato,Naoki Yoshida,Kaho Mochizuki,Hiroki Tsumoto,Yuri Miura

The Journal of experimental medicine 215:1853-1868 PubMed29930104

2018

Trim33 mediates the proinflammatory function of Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Tanaka,Yu Jiang,Gustavo J Martinez,Kentaro Tanaka,Xiaowei Yan,Tomohiro Kurosaki,Vesa Kaartinen,Xin-Hua Feng,Qiang Tian,Xiaohu Wang,Chen Dong

PloS one 9:e104323 PubMed25116007

2014

Activation of nuclear factor kappa B in the hepatic stellate cells of mice with schistosomiasis japonica.

Applications

Species

Mouse

Xing He,Guangbin Pu,Rui Tang,Dongmei Zhang,Weiqing Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H1.0 antibody [EPR6536]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

Product promise