Rabbit Recombinant Monoclonal H1.0 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (AB203946), expandable thumbnail](https://content.abcam.com/products/images/histone-h10-antibody-epr6536-bsa-and-azide-free-ab203946--western-blot-img138924.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (AB203946)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Expected |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Alternative names
H1F0, H1FV, H1-0, Histone H1.0, Histone H1', Histone H1(0)
Recommended products
Rabbit Recombinant Monoclonal H1.0 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6536
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab203946 is the carrier-free version of Anti-Histone H1.0 antibody [EPR6536] ab134914.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946), expandable thumbnail](https://content.abcam.com/products/images/histone-h10-antibody-epr6536-bsa-and-azide-free-ab203946--western-blot-img138924.jpg "Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)")
Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Histone H1.0 antibody [EPR6536] ab134914).
Lanes 1 - 3: Merged signal (red and green). Green - Anti-Histone H1.0 antibody [EPR6536] ab134914 observed at 30 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
Anti-Histone H1.0 antibody [EPR6536] ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Histone H1.0 antibody [EPR6536] ab134914 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Histone H1.0 antibody [EPR6536] (Anti-Histone H1.0 antibody [EPR6536] ab134914) at 1/1000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: H1F0 knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human H1F0 knockout A-431 cell line (Human H1F0 knockout A-431 cell line ab262478)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 30 kDa
Downloads
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