Rabbit Polyclonal H1.2 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 33 publications.
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- Life science alliance 6:2023The linker histone H1-BRCA1 axis is a crucial mediator of replication fork stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeryem Ozgencil,Arlinda Dullovi,Romane Catherine Christiane Higos,Zuzana Hořejší,Roberto BellelliPubMed 37364916
- Nature communications 12:34972021Site-specific ubiquitylation acts as a regulator of linker histone H1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Höllmüller,Simon Geigges,Marie L Niedermeier,Kai-Michael Kammer,Simon M Kienle,Daniel Rösner,Martin Scheffner,Andreas Marx,Florian StengelPubMed 34108453
- Journal of cell science 133:2020Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesImke K Mandemaker et. al.PubMed 32184266
- Nucleus (Austin, Tex.) 11:1-182020Hyperosmotic stress: chromatin phase separation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAda L Olins,Travis J Gould,Logan Boyd,Bettina Sarg,Donald E OlinsPubMed 31924112
- Cancer management and research 10:5825-5838201812-HETE facilitates cell survival by activating the integrin-linked kinase/NF-κB pathway in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Liu et. al.PubMed 30510451
- Molecular cell 72:250-262.e62018Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Clouaire et. al.PubMed 30270107
- The Journal of allergy and clinical immunology 142:959-9692018Genetic variants with gene regulatory effects are associated with diisocyanate-induced asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid I Bernstein et. al.PubMed 29969634
- Cell research 28:756-7702018Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiming Li et. al.PubMed 29844578
- Bio-protocol 8:e28212018A Method for Extracting the Nuclear Scaffold from the Chromatin Network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Chen,Boon Heng Dennis Teo,Jinhua LuPubMed 34286031
- The Journal of biological chemistry 293:2358-23692018The linker histone H1.2 is a novel component of the nucleolar organizer regions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Chen et. al.PubMed 29301938
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ICC/IF | WB | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Target data
Function
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Alternative names
H1F2, HIST1H1C, H1-2, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1
Recommended products
Rabbit Polyclonal H1.2 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 33 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunoprecipitation - Anti-Histone H1.2 antibody (ab17677)
Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit polyclonal to Histone H1.2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 35kDa; Histone H1.2
All lanes: Immunoprecipitation - Anti-Histone H1.2 antibody (ab17677)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 20min
Western blot - Anti-Histone H1.2 antibody (ab17677)
False colour image of Western blot: Anti-Histone H1.2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab17677 was shown to bind specifically to Histone H1.2. A band was observed at 32 kDa in wild-type HeLa cell lysates with no signal observed at this size in HIST1H1C knockout cell line Human HIST1H1C (Histone H1.2) knockout HeLa cell line ab261794 (knockout cell lysate Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate ab257218). To generate this image, wild-type and HIST1H1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HIST1H1C knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human HIST1H1C (Histone H1.2) knockout HeLa cell line (Human HIST1H1C (Histone H1.2) knockout HeLa cell line ab261794)
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 32 kDa
This image is courtesy of Dr Albert Jordan and Monica Sancho, Center for Genomic Regulation (CRG). Recombinant H1 isoforms courtesy of Dr Nicole Happel.Western blot - Anti-Histone H1.2 antibody (ab17677)
All lanes: Western blot - Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1: Recombinant histone H1
Lane 2: Recombinant histone H1.1
Lane 3: Recombinant histone H1.2
Lane 4: Recombinant histone H1.3
Lane 5: Recombinant histone H1.4
Lane 6: Recombinant histone H1.5
Predicted band size: 21 kDa
Observed band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody (ab17677)
ab17677 staining Histone H1.2 in HeLa cells. The cells were fixed with 100% methanol (5 min) at room temperature, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tritonn for 1h. The cells were then incubated with the antibody ab16766 at 0.1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature.
Western blot - Anti-Histone H1.2 antibody (ab17677)
All lanes: Western blot - Anti-Histone H1.2 antibody (ab17677) at 1 µg/mL
Lanes 1 and 3: Histone H1 Recombinant Protein at 0.1 µg
Lanes 2 and 4: Histone H1.2 (Human) - Recombinant Protein at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 29 kDa
Exposure time: 12min
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