Rabbit Polyclonal H1.3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human H1-3 aa 1-50.
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Publications
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- Cancer research 82:2887-29032022DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYabing Nan,Qingyu Luo,Xiaowei Wu,Shi Liu,Pengfei Zhao,Wan Chang,Aiping Zhou,Zhihua LiuPubMed 35731019
- Nucleic acids research 50:3892-39102022Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNúria Serna-Pujol,Mónica Salinas-Pena,Francesca Mugianesi,François Le Dily,Marc A Marti-Renom,Albert JordanPubMed 35380694
- Clinical epigenetics 11:1412019Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSylvain Garciaz et. al.PubMed 31606046
- Cell research 28:756-7702018Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiming Li et. al.PubMed 29844578
- Nucleic acids research 45:11622-116422017Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Izquierdo-Bouldstridge,Alberto Bustillos,Carles Bonet-Costa,Patricia Aribau-Miralbés,Daniel García-Gomis,Marc Dabad,Anna Esteve-Codina,Laura Pascual-Reguant,Sandra Peiró,Manel Esteller,Matthew Murtha,Lluís Millán-Ariño,Albert JordanPubMed 28977426
- The Journal of biological chemistry 290:7474-912015Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.
- Cancer research 74:6463-732014Histone h1.3 suppresses h19 noncoding RNA expression and cell growth of ovarian cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Medrzycki et. al.PubMed 25205099
- FEBS letters 588:2353-622014Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Michel Terme et. al.PubMed 24873882
- PLoS genetics 8:e10028792012Dynamic distribution of linker histone H1.5 in cellular differentiation.
- The Journal of biological chemistry 286:35347-572011Histone H1 variants are differentially expressed and incorporated into chromatin during differentiation and reprogramming to pluripotency.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 20% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H1-3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16402
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes H1.3 is present in many human cell lines. Cross-reacts with bands of approx. 52kDa and 45kDa in nuclear extracts of T47D cell line. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Alternative names
H1F3, HIST1H1D, H1-3, Histone H1.3, Histone H1c, Histone H1s-2
Recommended products
Rabbit Polyclonal H1.3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human H1-3 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 20% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H1-3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P16402
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
This image is courtesy of Dr Albert Jordan, Center for Genomic Regulation (CRG).Western blot - Anti-Histone H1.3 antibody (ab24174)
Rabbit polyclonal to Histone H1.3 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line. Notice that extra bands are present/detected in the chromatin preparation.
All lanes: Western blot - Anti-Histone H1.3 antibody (ab24174)
Predicted band size: 22 kDa
This image is courtesy of Katia AncelinImmunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody (ab24174)
Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab24174 (1/200) for Histone H1.3 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody (ab24174)
IHC image of ab24174 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24174, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Product protocols
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