JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB61177

Anti-Histone H1.3 + Histone H1.4 antibody

5

(2 Reviews)

|

(15 Publications)

Rabbit Polyclonal H1.3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human H1-3 aa 1-50.

View Alternative Names

H1F3, HIST1H1D, H1-3, Histone H1.3, Histone H1c, Histone H1s-2, H1F4, HIST1H1E, H1-4, Histone H1.4, Histone H1b, Histone H1s-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)

Paraffin-embedded human colon carcinoma tissue stained for Histone H1.3 + Histone H1.4 with ab61177 at 1/50 dilution in immunohistochemical analysis.

The sample in the right-hand panel is incubated with the immunizing peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)

ab61177 at 1/500 - 1/1000 dilution staining Histone H1.3 + Histone H1.4 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)

IHC image of ab61177 staining Histone H1.3 + Histone H1.4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)
  • WB

Supplier Data

Western blot - Anti-Histone H1.3 + Histone H1.4 antibody (AB61177)

All lanes:

Western blot - Anti-Histone H1.3 + Histone H1.4 antibody (ab61177)

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HepG2 whole cell lysate with blocking peptide

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H1-3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16402

Specificity

This antibody detects both Histone H1.3 and Histone H1.4.

style
left-column

Complementary Products

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB134914

Anti-Histone H1.0 antibody [EPR6536]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

  • 5
  • 1
Primary Antibodies

AB183736

Anti-Histone H1.3 antibody [EPR12683]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] (AB183736)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
See full target information H1-3
style
left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:286-294 PubMed39420835

2024

G6PC3 is involved in spermatogenesis by maintaining meiotic sex chromosome inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuming Cao,Shengnan Wang,Liyang Li,Wenwen Li,Yan Liang,Fei Ao,Zexiao Wei,Li Wang

Nucleic acids research 51:4791-4813 PubMed36919611

2023

GCNA is a histone binding protein required for spermatogonial stem cell maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Ribeiro,Gerry P Crossan

European heart journal open 2:oeac047 PubMed36117952

2022

Vascular senescence in progeria: role of endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Xu,Anahita Mojiri,Luay Boulahouache,Elisa Morales,Brandon K Walther,John P Cooke

Molecular cell 82:106-122.e9 PubMed34875212

2021

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.

Applications

Unspecified application

Species

Unspecified reactive species

Daoud Sheban,Tom Shani,Roey Maor,Alejandro Aguilera-Castrejon,Nofar Mor,Bernardo Oldak,Merav D Shmueli,Avital Eisenberg-Lerner,Jonathan Bayerl,Jakob Hebert,Sergey Viukov,Guoyun Chen,Assaf Kacen,Vladislav Krupalnik,Valeriya Chugaeva,Shadi Tarazi,Alejandra Rodríguez-delaRosa,Mirie Zerbib,Adi Ulman,Solaiman Masarwi,Meital Kupervaser,Yishai Levin,Efrat Shema,Yael David,Noa Novershtern,Jacob H Hanna,Yifat Merbl

eLife 10: PubMed34018922

2021

Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Zhang,Li Zou,Lanxia Meng,Min Xiong,Lina Pan,Guiqin Chen,Yongfa Zheng,Jing Xiong,Zhihao Wang,Duc M Duong,Zhaohui Zhang,Xuebing Cao,Tao Wang,Li Tang,Keqiang Ye,Zhentao Zhang

Nature communications 12:626 PubMed33504790

2021

LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate.

Applications

Unspecified application

Species

Unspecified reactive species

Run Jin,Samantha Klasfeld,Yang Zhu,Meilin Fernandez Garcia,Jun Xiao,Soon-Ki Han,Adam Konkol,Doris Wagner

Cells 8: PubMed31035509

2019

Pinocembrin Protects from AGE-Induced Cytotoxicity and Inhibits Non-Enzymatic Glycation in Human Insulin.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Borriello,Clara Iannuzzi,Ivana Sirangelo

Journal of cellular physiology 234:19715-19727 PubMed30982984

2019

Overexpressed long noncoding RNA CPS1-IT alleviates pulmonary arterial hypertension in obstructive sleep apnea by reducing interleukin-1β expression via HIF1 transcriptional activity.

Applications

Unspecified application

Species

Unspecified reactive species

Zeming Zhang,Zheng Li,Yancun Wang,Li Wei,Hao Chen

Cell discovery 4:61 PubMed30455981

2018

Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Therese El-Daher,Nicolas Cagnard,Marine Gil,Marie Chansel Da Cruz,Claire Leveau,Fernando Sepulveda,Mohammed Zarhrate,Frédéric Tores,Patricia Legoix,Sylvain Baulande,Jean Pierre de Villartay,Geneviève Almouzni,Jean-Pierre Quivy,Alain Fischer,Geneviève de Saint Basile

The Journal of infectious diseases 219:1418-1429 PubMed30346568

2018

Parvovirus B19V Nonstructural Protein NS1 Induces Double-Stranded Deoxyribonucleic Acid Autoantibodies and End-Organ Damage in Nonautoimmune Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kanoktip Puttaraksa,Heidi Pirttinen,Kati Karvonen,Jonna Nykky,Stanley J Naides,Leona Gilbert
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com