JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326647

Anti-Histone H1.4 (phospho T17) antibody [SR1513]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal H1.4 phospho T17 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

H1F4, HIST1H1E, H1-4, Histone H1.4, Histone H1b, Histone H1s-4, H1T17p

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.4 (phospho T17) antibody [SR1513] (AB326647)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.4 (phospho T17) antibody [SR1513] (AB326647)

Immunohistochemical analysis of paraffin-embedded mouse colon, using ab326647.

Western blot - Anti-Histone H1.4 (phospho T17) antibody [SR1513] (AB326647)
  • WB

Supplier Data

Western blot - Anti-Histone H1.4 (phospho T17) antibody [SR1513] (AB326647)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Hep-G2 cell lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 22 kDa
Observed band size : 30 kDa
Exposure time : 6 seconds

All lanes:

Western blot - Anti-Histone H1.4 (phospho T17) antibody [SR1513] (ab326647) at 1/1000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

Hep-G2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 30 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1513

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed : 35581345, PubMed : 40240600). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed : 35581345, PubMed : 40240600, PubMed : 40516528). Ability to associate with nucleosomes and compact chromatin depends on linker DNA length and trajectory (PubMed : 35581345). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (PubMed : 40240600).
See full target information H1-4 pT17
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com