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AB203624

Anti-Histone H1.4 (phospho T18) antibody

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(1 Publication)

Rabbit Polyclonal H1.4 phospho T18 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H1-4 pT18 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

H1F4, HIST1H1E, H1-4, Histone H1.4, Histone H1b, Histone H1s-4, H1T18p

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.4 (phospho T18) antibody (AB203624)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.4 (phospho T18) antibody (AB203624)

Immunohistochemical analysis of formalin fixed paraffin embedded rat brain labeling Histone H1.4 (phospho T18) with ab203624 at 1/200 followed by conjugation to the secondary antibody and DAB staining

Western blot - Anti-Histone H1.4 (phospho T18) antibody (AB203624)
  • WB

Supplier Data

Western blot - Anti-Histone H1.4 (phospho T18) antibody (AB203624)

All lanes:

Western blot - Anti-Histone H1.4 (phospho T18) antibody (ab203624) at 1/200 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Mouse cerebrum lysate

Predicted band size: 21 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human H1-4 pT18 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10412

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe use at 1/50 - 1/200</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed : 35581345, PubMed : 40240600). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed : 35581345, PubMed : 40240600, PubMed : 40516528). Ability to associate with nucleosomes and compact chromatin depends on linker DNA length and trajectory (PubMed : 35581345). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (PubMed : 40240600).
See full target information H1-4 pT18

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Critical reviews in eukaryotic gene expression 32:11-20 PubMed35997114

2022

LncRNA PCED1B-AS1 Upregulation in Hepatocellular Carcinoma and Regulation of the miR-10a/BCL6 Axis to Promote Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Ding,Juan He,Weili Xiao,Zhihong Ren,Wanting Gao
View all publications

Product promise

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