AB24175

Anti-Histone H1.5 antibody

Name: Anti-Histone H1.5 antibody (ab24175) | Abcam
Brand: Abcam Limited
SKU: AB24175
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal H1.5 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human H1-5 aa 1-50.

View Alternative Names

H1F5, HIST1H1B, H1-5, Histone H1.5, Histone H1a, Histone H1b, Histone H1s-3

1 Images

Unknown

Western blot - Anti-Histone H1.5 antibody (AB24175)

All lanes : Histone H1.5 antibody (ab24175)

Lane 1 : Chromatin preparation from a breast cancer cell line
Lane 2 : recombinant human H1.0
Lane 3 : recombinant human H1.1
Lane 4 : recombinant human H1.2
Lane 5 : recombinant human H1.3
Lane 6 : recombinant human H1.4
Lane 7 : recombinant human H1.5

Panel B is a silver stain of the gel in panel A.

All lanes:

Western blot - Anti-Histone H1.5 antibody (ab24175)

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human H1-5 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P16401

Specificity

This antibody is specific for Histone H1.5. Reacts with histone H1 acid-extracted from cell lines such as breast cancer cell line T47D. Also recognizes H1 in a nuclear extract or chromatin preparation. H1.5 is present in many human cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 8% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

See full target information H1-5

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:3892-3910 PubMed35380694

2022

Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Núria Serna-Pujol,Mónica Salinas-Pena,Francesca Mugianesi,François Le Dily,Marc A Marti-Renom,Albert Jordan

Cell 184:3163-3177.e21 PubMed33964209

2021

Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Cui,Kasturi Chakraborty,Xu Anna Tang,Guolin Zhou,Kelly Q Schoenfelt,Kristen M Becker,Alexandria Hoffman,Ya-Fang Chang,Ariane Blank,Catherine A Reardon,Hilary A Kenny,Tomas Vaisar,Ernst Lengyel,Geoffrey Greene,Lev Becker

Nucleic acids research 45:11622-11642 PubMed28977426

2017

Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Izquierdo-Bouldstridge,Alberto Bustillos,Carles Bonet-Costa,Patricia Aribau-Miralbés,Daniel García-Gomis,Marc Dabad,Anna Esteve-Codina,Laura Pascual-Reguant,Sandra Peiró,Manel Esteller,Matthew Murtha,Lluís Millán-Ariño,Albert Jordan

Epigenetics & chromatin 10:29 PubMed28539972

2017

Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Liao,Craig A Mizzen

Scientific reports 5:16495 PubMed26559910

2015

NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Holmberg Olausson,Tamador Elsir,Kaveh Moazemi Goudarzi,Monica Nistér,Mikael S Lindström

Nucleic acids research 43:10200-12 PubMed26319017

2015

Nucleosome compaction facilitates HP1γ binding to methylated H3K9.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Mishima,Chanika D Jayasinghe,Kai Lu,Junji Otani,Masahiro Shirakawa,Toru Kawakami,Hironobu Kimura,Hironobu Hojo,Peter Carlton,Shoji Tajima,Isao Suetake

The Journal of biological chemistry 290:7474-91 PubMed25645921

2015

Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.

Applications

Species

Human

Regina Mayor,Andrea Izquierdo-Bouldstridge,Lluís Millán-Ariño,Alberto Bustillos,Cristina Sampaio,Neus Luque,Albert Jordan

The Journal of biological chemistry 289:34601-19 PubMed25349213

2014

Loss of nucleolar histone chaperone NPM1 triggers rearrangement of heterochromatin and synergizes with a deficiency in DNA methyltransferase DNMT3A to drive ribosomal DNA transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Holmberg Olausson,Monica Nistér,Mikael S Lindström

Cancer research 74:6463-73 PubMed25205099

2014

Histone h1.3 suppresses h19 noncoding RNA expression and cell growth of ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Medrzycki,Yunzhe Zhang,Weijia Zhang,Kaixiang Cao,Chenyi Pan,Nathalie Lailler,John F McDonald,Eric E Bouhassira,Yuhong Fan

FEBS letters 588:2353-62 PubMed24873882

2014

Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Michel Terme,Lluís Millán-Ariño,Regina Mayor,Neus Luque,Andrea Izquierdo-Bouldstridge,Alberto Bustillos,Cristina Sampaio,Jordi Canes,Isaura Font,Núria Sima,Mónica Sancho,Laura Torrente,Sonia Forcales,Alicia Roque,Pere Suau,Albert Jordan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H1.5 antibody

Western blot - Anti-Histone H1.5 antibody (AB24175)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

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