Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Rabbit Recombinant Monoclonal H2A acetyl K13 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (AB249960), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	PepArr
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information H2AC4 acetyl K13

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H2AFM, HIST1H2AB, H2AC8, H2AFA, HIST1H2AE, H2AC4, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AK13ac

Recommended products

Rabbit Recombinant Monoclonal H2A acetyl K13 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr and reacts with Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR17476(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab249960 is the carrier-free version of Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)
This data was developed using Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316) at 1/1000 dilution
Lane 1: Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 500ng/ml TSA for 4 hours whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 5s
Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)
This data was developed using Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 500ng/ml TSA for 4 hours whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1s
Peptide Array - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)
This data was developed using Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316, the same antibody clone in a different buffer formulation.
Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)
This data was developed using Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 500ng/ml TSA for 4 hours, labeling Histone H2A (acetyl K13) with Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). The results show nuclear staining on TSA (500ng/ml for 4hr) treated HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^®594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^®594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)
This data was developed using Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells, untreated or treated with 500ng/ml TSA for 4 hours, labeling Histone H2A (acetyl K13) with Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). The results show nuclear staining on TSA (500ng/ml for 4hr) treated NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] ab177316 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 secondary antibody at 1/1000 dilution.

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Product protocols

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free

Images

Key facts

Immunogen

Reactivity data

ICC/IF

TestedTested

WB

TestedTested

PepArr

TestedTested

ExpectedExpected

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Product promise

Tested

Expected

Predicted

Not recommended

5 product images

Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Peptide Array - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] - BSA and Azide free (ab249960)

Downloads

Product protocols

Tested
Tested

Tested
Tested

Tested
Tested

Expected
Expected