AB1764

Anti-Histone H2A (acetyl K5) antibody

Name: Anti-Histone H2A (acetyl K5) antibody (ab1764) | Abcam
Brand: Abcam Limited
SKU: AB1764
Price: 565 USD
Availability: InStock
Rating: 4 (10 reviews)

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What is this?

(10 Reviews)

(13 Publications)

Rabbit Polyclonal H2A acetyl K5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human H2AC4 acK5 conjugated to Ovalbumin.

View Alternative Names

H2AFM, HIST1H2AB, H2AC8, H2AFA, HIST1H2AE, H2AC4, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AK5ac

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody (AB1764)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody (AB1764)

IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human H2AC4 acK5 conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P04908

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2AC4 acK5

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1611226 PubMed40530334

2025

Induced neural stem cells ameliorate blood-brain barrier injury by modulating the calcium signaling pathway of astrocyte in cerebral ischemia-reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyun Liang,Chuanshang Cao,Ningmei Liu,Dongmei Chen,Ting Liu,Haibin Ma,Jiaxin Liu,Taojuan Wu,Jianguo Niu

Epigenetics & chromatin 9:44 PubMed27777629

2016

An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in males.

Applications

ICC/IF

Species

Unspecified reactive species

Alyssa C Lau,Kevin P Zhu,Elizabeth A Brouhard,Michael B Davis,Györgyi Csankovszki

eLife 5: PubMed27244239

2016

Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity.

Applications

Species

Unspecified reactive species

Kotaro Shirakawa,Lan Wang,Na Man,Jasna Maksimoska,Alexander W Sorum,Hyung W Lim,Intelly S Lee,Tadahiro Shimazu,John C Newman,Sebastian Schröder,Melanie Ott,Ronen Marmorstein,Jordan Meier,Stephen Nimer,Eric Verdin

Nature medicine 21:1154-62 PubMed26390242

2015

Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Won Min,Xu Chen,Tara E Tracy,Yaqiao Li,Yungui Zhou,Chao Wang,Kotaro Shirakawa,S Sakura Minami,Erwin Defensor,Sue Ann Mok,Peter Dongmin Sohn,Birgit Schilling,Xin Cong,Lisa Ellerby,Bradford W Gibson,Jeffrey Johnson,Nevan Krogan,Mehrdad Shamloo,Jason Gestwicki,Eliezer Masliah,Eric Verdin,Li Gan

Molecular cell 59:502-11 PubMed26212453

2015

An Interactive Database for the Assessment of Histone Antibody Specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Scott B Rothbart,Bradley M Dickson,Jesse R Raab,Adrian T Grzybowski,Krzysztof Krajewski,Angela H Guo,Erin K Shanle,Steven Z Josefowicz,Stephen M Fuchs,C David Allis,Terry R Magnuson,Alexander J Ruthenburg,Brian D Strahl

Cell cycle (Georgetown, Tex.) 12:2033-41 PubMed23777805

2013

Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Ruozhen Hu,Edward Wang,Guang Peng,Hui Dai,Shiaw-Yih Lin

Cell 148:335-48 PubMed22244452

2012

Waves of retrotransposon expansion remodel genome organization and CTCF binding in multiple mammalian lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Schmidt,Petra C Schwalie,Michael D Wilson,Benoit Ballester,Angela Gonçalves,Claudia Kutter,Gordon D Brown,Aileen Marshall,Paul Flicek,Duncan T Odom

PloS one 3:e3650 PubMed18985155

2008

Analysis of Myc-induced histone modifications on target chromatin.

Applications

ChIP

Species

Unspecified reactive species

Francesca Martinato,Matteo Cesaroni,Bruno Amati,Ernesto Guccione

Nature 452:492-6 PubMed18337721

2008

SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin.

Applications

Species

Human

Eriko Michishita,Ronald A McCord,Elisabeth Berber,Mitomu Kioi,Hesed Padilla-Nash,Mara Damian,Peggie Cheung,Rika Kusumoto,Tiara L A Kawahara,J Carl Barrett,Howard Y Chang,Vilhelm A Bohr,Thomas Ried,Or Gozani,Katrin F Chua

FEBS letters 581:5275-81 PubMed17961556

2007

Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair defects post exposure to gamma ionizing radiation.

Applications

Species

Mouse

Sandra N Garcia,Bhakti M Kirtane,Andrej J Podlutsky,Olivia M Pereira-Smith,Kaoru Tominaga

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A (acetyl K5) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody (AB1764)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (13)

Product promise