Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
Images
![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--western-blot-img45177.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)")
![Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunoprecipitation-img9432.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunocytochemistry-immunofluorescence-img93003.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133210.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41150.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)")
Publications
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- Nature communications 14:41032023Epigenetic mechanisms to propagate histone acetylation by p300/CBP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaki Kikuchi,Satoshi Morita,Masatoshi Wakamori,Shin Sato,Tomomi Uchikubo-Kamo,Takehiro Suzuki,Naoshi Dohmae,Mikako Shirouzu,Takashi UmeharaPubMed 37460559
- PLoS genetics 16:e10091852020Opposing functions of Fng1 and the Rpd3 HDAC complex in H4 acetylation in Fusarium graminearum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Jiang et. al.PubMed 33137093
- Neural regeneration research 15:277-2842019Neuroprotective mechanism of TMP269, a selective class IIA histone deacetylase inhibitor, after cerebral ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Su,Dan Liang,Shen-Yi Kuang,Qiang Dong,Xiang Han,Zheng WangPubMed 31552900
- PLoS genetics 15:e10083162019The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Gómez-H et. al.PubMed 31437213
- eLife 7:2018A effector recruits a host cytoplasmic transacetylase into nuclear speckles to enhance plant susceptibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyang Li et. al.PubMed 30346270
- Toxicology letters 295:270-2762018Poly (ADP-ribose) glycohydrolase silencing-mediated maintenance of H2A and downregulation of H2AK9me protect human bronchial epithelial cells from benzo(a)pyrene-induced carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuoying Zeng et. al.PubMed 29981922
- Molecular cell 59:502-112015An Interactive Database for the Assessment of Histone Antibody Specificity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott B Rothbart et. al.PubMed 26212453
- Chemistry & biology 22:849-612015Non-canonical Bromodomain within DNA-PKcs Promotes DNA Damage Response and Radioresistance through Recognizing an IR-Induced Acetyl-Lysine on H2AX.
- World journal of gastroenterology 21:6665-742015Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Zhou,Jie Xia,Yong-Jie Zhou,Jun Wan,Li Li,Ji Bao,Yu-Jun Shi,Hong BuPubMed 26074705
- The Journal of biological chemistry 288:18271-822013Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Expected | Tested | Tested | Tested |
| Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200 - 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AFD, HIST1H2AK, H2AC16, HIST1H2AL, H2AFI, H2AC17, H2AC15, HIST1H2AI, H2AFC, H2AC13, HIST1H2AG, H2AFN, HIST1H2AM, H2AFP, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl, H2AK5ac
Recommended products
Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP856Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--western-blot-img45177.jpg "Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
All lanes: Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution
All lanes: Mouse cortex whole tissue lysate at 20 µg
SecondaryAll lanes: HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 1min
![Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunoprecipitation-img9432.jpg "Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
ab45152 immunoprecipitating Histone H2A. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/10000.
Lane 1: Mouse brain whole cell lysate
Lane 2: Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab45152 in Mouse brain whole cell lysateAll lanes: Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
Predicted band size: 14 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunocytochemistry-immunofluorescence-img93003.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-EEF2/Elongation factor 2 antibody [EP880Y] ab75748 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133210.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41150.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--western-blot-img56988.jpg "Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
All lanes: Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution
Lane 1: C6 cell lysate
Lane 2: C6 + TSA membrane
Predicted band size: 14 kDa
Observed band size: 14 kDa, 34 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81572.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--western-blot-img56988.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-acetyl-k5-antibody-ep856y-ab45152--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81572.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
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