AB45152

Anti-Histone H2A (acetyl K5) antibody [EP856Y]

Name: Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) | Abcam
Brand: Abcam Limited
SKU: AB45152
Price: 610 USD
Availability: InStock
Rating: 4 (14 reviews)

RabMAb
Recombinant
What is this?

(14 Reviews)

(16 Publications)

Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl, H2AK5ac

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells treated and untreated with TSA500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Lab

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

ab45152 immunoprecipitating Histone H2A. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate

All lanes:

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

Predicted band size: 14 kDa

false

AbReview40090****

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

All lanes:

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution

All lanes:

Mouse cortex whole tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

All lanes:

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution

Lane 1:

C6 cell lysate

Lane 2:

C6 + TSA membrane

Predicted band size: 14 kDa

Observed band size: 14 kDa,34 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H2A (acetyl K5) antibody [EP856Y]
Carrier free

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP856Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": "" }, "Caenorhabditis elegans": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2AC11 acetyl K5

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1566567 PubMed40213392

2025

Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Liu,Jun Zhang,Jingdan Zhang,Yucheng Yuan,Zhiqing Liu,Sixian Chen,Kaifeng Chen,Li Dong,Zhiyuan Cheng,Yinan Zhang,Meiyu Geng,Minjia Tan,Wensi Zhao,Dong Xie

PloS one 19:e0309598 PubMed39240880

2024

Curcumin alleviates heatstroke-induced liver injury in dry-heat environments by inhibiting the expression of NF-κB, iNOS, and ICAM-1 in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Yang,Liang Xia,Caifu Shen,Jiajia Li,Xiang Dong,Jiangwei Liu

Reproduction (Cambridge, England) 166:285-297 PubMed37490350

2023

A long non-coding RNA essential for early embryonic development improves somatic cell nuclear transfer somatic cell nuclear transfer efficiency in goats.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Jin,Lu Zhao,Hanwen Yang,Jianglin Zhao,Hongwei Ma,Yanzhi Chen,Jingcheng Zhang,Yan Luo,Yong Zhang,Jun Liu

Nature communications 14:4103 PubMed37460559

2023

Epigenetic mechanisms to propagate histone acetylation by p300/CBP.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Kikuchi,Satoshi Morita,Masatoshi Wakamori,Shin Sato,Tomomi Uchikubo-Kamo,Takehiro Suzuki,Naoshi Dohmae,Mikako Shirouzu,Takashi Umehara

Nature metabolism 4:693-710 PubMed35760868

2022

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Emily J Kay,Karla Paterson,Carla Riera-Domingo,David Sumpton,J Henry M Däbritz,Saverio Tardito,Claudia Boldrini,Juan R Hernandez-Fernaud,Dimitris Athineos,Sandeep Dhayade,Ekaterina Stepanova,Enio Gjerga,Lisa J Neilson,Sergio Lilla,Ann Hedley,Grigorios Koulouras,Grace McGregor,Craig Jamieson,Radia Marie Johnson,Morag Park,Kristina Kirschner,Crispin Miller,Jurre J Kamphorst,Fabricio Loayza-Puch,Julio Saez-Rodriguez,Massimiliano Mazzone,Karen Blyth,Michele Zagnoni,Sara Zanivan

PLoS genetics 16:e1009185 PubMed33137093

2020

Opposing functions of Fng1 and the Rpd3 HDAC complex in H4 acetylation in Fusarium graminearum.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Jiang,Aliang Xia,Meng Ye,Jingyi Ren,Dongao Li,Huiquan Liu,Qinhu Wang,Ping Lu,Chunlan Wu,Jin-Rong Xu,Cong Jiang

Neural regeneration research 15:277-284 PubMed31552900

2019

Neuroprotective mechanism of TMP269, a selective class IIA histone deacetylase inhibitor, after cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Su,Dan Liang,Shen-Yi Kuang,Qiang Dong,Xiang Han,Zheng Wang

PLoS genetics 15:e1008316 PubMed31437213

2019

The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Gómez-H,Natalia Felipe-Medina,Yazmine B Condezo,Rodrigo Garcia-Valiente,Isabel Ramos,José Angel Suja,José Luis Barbero,Ignasi Roig,Manuel Sánchez-Martín,Dirk G de Rooij,Elena Llano,Alberto M Pendas

eLife 7: PubMed30346270

2018

A effector recruits a host cytoplasmic transacetylase into nuclear speckles to enhance plant susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyang Li,Haonan Wang,Maofeng Jing,Jinyi Zhu,Baodian Guo,Yang Wang,Yachun Lin,Han Chen,Liang Kong,Zhenchuan Ma,Yan Wang,Wenwu Ye,Suomeng Dong,Brett Tyler,Yuanchao Wang

Toxicology letters 295:270-276 PubMed29981922

2018

Poly (ADP-ribose) glycohydrolase silencing-mediated maintenance of H2A and downregulation of H2AK9me protect human bronchial epithelial cells from benzo(a)pyrene-induced carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoying Zeng,Hailong Liu,Jianhui Yuan,Xiaohu Ren,Yanxia Deng,Wenjuan Dai,Yue Wu,Yun Huang,Ruixue Huang,Jiaofeng Liu,Haiyan Huang,Jian'an Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A (acetyl K5) antibody [EP856Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (AB45152)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

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