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AB247302

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.

View Alternative Names

H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl, H2AK5ac

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • IP

Lab

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

ab45152 immunoprecipitating Histone H2A. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate

All lanes:

Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (<a href='/en-us/products/primary-antibodies/histone-h2a-acetyl-k5-antibody-ep856y-ab45152'>ab45152</a>)

Predicted band size: 14 kDa

false

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)
  • WB

Unknown

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (AB247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (<a href='/en-us/products/primary-antibodies/histone-h2a-acetyl-k5-antibody-ep856y-ab45152'>ab45152</a>) at 1/100000 dilution

Lane 1:

C6 cell lysate

Lane 2:

C6 + TSA membrane

Predicted band size: 14 kDa

Observed band size: 14 kDa,34 kDa

false

  • Unconjugated

    Anti-Histone H2A (acetyl K5) antibody [EP856Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H2A (acetyl K5) antibody [EP856Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP856Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB1790

Anti-Histone H2B antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H2B antibody - ChIP Grade (AB1790)

  • 5
  • 28
Primary Antibodies

AB10158

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

  • 4
  • 21
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Caenorhabditis elegans": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab247302 is the carrier-free version of ab45152.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H2AC11 acetyl K5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com