Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.
pH: 7.2 - 7.4
Constituents: PBS
IP | WB | IHC-P | ICC/IF | |
---|---|---|---|---|
Human | Expected | Expected | Tested | Expected |
Mouse | Tested | Expected | Tested | Expected |
Rat | Expected | Tested | Tested | Tested |
Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Caenorhabditis elegans | Dilution info - | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl, H2AK5ac
Rabbit Recombinant Monoclonal H2A acetyl K5 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab247302 is the carrier-free version of Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/10000.Lane 1: Mouse brain whole cell lysate
Lane 2: Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 in Mouse brain whole cell lysate
All lanes: Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152)
Predicted band size: 14 kDa
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-EEF2/Elongation factor 2 antibody [EP880Y] ab75748 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.Negative control 1: PBS in place of primary antibody.
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.Negative control 1: PBS in place of primary antibody.
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152) at 1/100000 dilution
Lane 1: C6 cell lysate
Lane 2: C6 + TSA membrane
Predicted band size: 14 kDa
Observed band size: 14 kDa, 34 kDa
This data was developed using Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152, the same antibody clone in a different buffer formulation.Anti-Histone H2A (acetyl K5) antibody [EP856Y] ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.Negative control 1: PBS in place of primary antibody.
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