AB242365

Anti-Histone H2A antibody [CL5039]

Name: Anti-Histone H2A antibody [CL5039] (ab242365) | Abcam
Brand: Abcam Limited
SKU: AB242365
Price: 635 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal H2A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H2AC11 aa 1-50.

View Alternative Names

H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Paraffin-embedded human testis tissue stained for Histone H2A using ab242365 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Paraffin-embedded human kidney tissue stained for Histone H2A using ab242365 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Paraffin-embedded human small intestine tissue stained for Histone H2A using ab242365 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Negative control :

Paraffin-embedded human cerebral cortex tissue stained for Histone H2A using ab242365 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H2A antibody [CL5039] (AB242365)

Recombinant human proteins.

All lanes:

Western blot - Anti-Histone H2A antibody [CL5039] (ab242365) at 1 µg/mL

Lane 1:

H2A

Lane 2:

H2A.X

Lane 3:

H2A.Z

Lane 4:

mH2A.1

Lane 5:

mH2A.2

Lane 6:

H2B

Lane 7:

Lane 8:

Predicted band size: 14 kDa

false

Supplier Data

Western blot - Anti-Histone H2A antibody [CL5039] (AB242365)

All lanes:

Western blot - Anti-Histone H2A antibody [CL5039] (ab242365) at 1 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Predicted band size: 14 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5039

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human H2AC11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P0C0S8

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2AC11

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35409271

2022

Dual Inhibition of H3K9me2 and H3K27me3 Promotes Tumor Cell Senescence without Triggering the Secretion of SASP.

Applications

Unspecified application

Species

Unspecified reactive species

Na Zhang,Mengjie Shang,Hongxin Li,Lan Wu,Meichen Dong,Baiqu Huang,Jun Lu,Yu Zhang

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A antibody [CL5039]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [CL5039] (AB242365)

Western blot - Anti-Histone H2A antibody [CL5039] (AB242365)

Western blot - Anti-Histone H2A antibody [CL5039] (AB242365)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise