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Rabbit Recombinant Monoclonal H2A antibody. Suitable for ChIC/CUT&RUN-seq, ChIP, WB, PepArr, ICC/IF, IHC-P and reacts with Human, Rat, Mouse, Synthetic peptide samples. Cited in 15 publications.


Images

ChIP - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (AB177308), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (AB177308), expandable thumbnail
  • Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (AB177308), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (AB177308), expandable thumbnail
  • Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (AB177308), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqChIPWBPepArrICC/IFIHC-P
Human
Tested
Tested
Tested
Expected
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Expected
Expected
Expected
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

2 µg

Notes

-

Expected
Expected

Species

Rat

Dilution info

2 µg

Notes

-

Species

Mouse

Dilution info

2 µg

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2 µg for 25.00000 µg chromatin

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H2A antibody. Suitable for ChIC/CUT&RUN-seq, ChIP, WB, PepArr, ICC/IF, IHC-P and reacts with Human, Rat, Mouse, Synthetic peptide samples. Cited in 15 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17470

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • ChIP - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    ChIP - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab177308 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIC/CUT&RUN sequencing - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ChIC/CUT&RUN pAG-MNase ab285373, 2.5 x 10^5 HeLa cells and 2μg of ab177308 [EPR17470]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads.
    Additional screenshots of mapped reads can be downloaded here.

  • Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308) at 1/5000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A with ab177308 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab177308 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308) at 1/1000 dilution

    Lane 1: mouse brain lysate at 10 µg

    Lane 2: mouse heart lysate at 10 µg

    Lane 3: mouse kidney lysate at 10 µg

    Lane 4: mouse spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

    Exposure time: 1s

  • Peptide Array - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Peptide Array - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    ab177308 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of Human colon tissue was observed.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.


    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of mouse colon tissue was observed.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution..

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2A using ab177308 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstained with Hematoxylin. Nucleus staining on epithelial cells of rat colon tissue was observed.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ChIC/CUT&RUN sequencing - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H2A antibody [EPR17470] - ChIP Grade (ab177308)

    ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 2 µg of ab177308 [EPR17470]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com