Anti-Histone H2A antibody [EPR18089] - ChIP Grade

3 product images

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  ChIP - Anti-Histone H2A antibody [EPR18089] - ChIP Grade (ab188312)

  Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab188312 (blue), and 20μl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

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  Western blot - Anti-Histone H2A antibody [EPR18089] - ChIP Grade (ab188312)

  Blocking/Dilution buffer: 5% BSA/TBST.

  All lanes: Western blot - Anti-Histone H2A antibody [EPR18089] - ChIP Grade (ab188312) at 1/500 dilution

  All lanes: Saccharomyces cerevisiae lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 14 kDa

  Observed band size: 14 kDa

  Exposure time: 30s

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  Peptide Array - Anti-Histone H2A antibody [EPR18089] - ChIP Grade (ab188312)

  ab188312 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
  Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
  The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.