AB4175

Anti-Histone H2A-Bbd antibody

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(3 Publications)

Rabbit Polyclonal H2A.B antibody. Suitable for ICC, WB and reacts with Mouse, Chicken, Human samples. Cited in 3 publications.

View Alternative Names

H2AFB2, H2AB3, H2ABBD, H2AFB, H2AFB3, H2AB2, Histone H2A-Bbd type 2/3, H2A Barr body-deficient, H2A.Bbd

2 Images

Collaborator

Immunocytochemistry - Anti-Histone H2A-Bbd antibody (AB4175)

Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.

Supplier Data

Western blot - Anti-Histone H2A-Bbd antibody (AB4175)

H2A-Bbd (ab4175)

CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).

The primary antibody dilution was 1 : 500 and the secondary was 1 : 3000.

As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.

All lanes:

Western blot - Anti-Histone H2A-Bbd antibody (ab4175)

Predicted band size: 13 kDa

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Juan Ausio

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Chicken, Human

Applications

WB, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Known to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein." }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "3 µg/mL", "ICC-species-notes": "Staining may not be specific for H2A.Bbd (see image).", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis.

See full target information H2AB2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genetics and molecular research : GMR 13:5929-39 PubMed25117351

2014

Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development.

Applications

Unspecified application

Species

Unspecified reactive species

B J Wu,F L Dong,X S Ma,X G Wang,F Lin,H L Liu

Development (Cambridge, England) 137:3785-94 PubMed20943707

2010

Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Buhe Nashun,Masashi Yukawa,Honglin Liu,Tomohiko Akiyama,Fugaku Aoki

Human molecular genetics 18:2567-74 PubMed19380460

2009

Chromatin analysis of occluded genes.

Applications

ChIP

Species

Human

Jae Hyun Lee,Jedidiah Gaetz,Branimir Bugarija,Croydon J Fernandes,Gregory E Snyder,Eliot C Bush,Bruce T Lahn

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