Anti-Histone H2A-Bbd antibody
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(3 Publications)
Rabbit Polyclonal H2A.B antibody. Suitable for ICC, WB and reacts with Mouse, Chicken, Human samples. Cited in 3 publications.
View Alternative Names
H2AFB2, H2AB3, H2ABBD, H2AFB, H2AFB3, H2AB2, Histone H2A-Bbd type 2/3, H2A Barr body-deficient, H2A.Bbd
- ICC
Collaborator
Immunocytochemistry - Anti-Histone H2A-Bbd antibody (AB4175)
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
This image is courtesy of Petra Hajkova, University of Cambridge
- WB
Supplier Data
Western blot - Anti-Histone H2A-Bbd antibody (AB4175)
H2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1 : 500 and the secondary was 1 : 3000.
As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
All lanes:
Western blot - Anti-Histone H2A-Bbd antibody (ab4175)
Predicted band size: 13 kDa
false
Juan Ausio
Reactivity data
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Appropriate long-term storage conditions
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Publications (3)
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Genetics and molecular research : GMR 13:5929-39 PubMed25117351
2014
Applications
Unspecified application
Species
Unspecified reactive species
Development (Cambridge, England) 137:3785-94 PubMed20943707
2010
Applications
WB, ICC/IF
Species
Mouse, Mouse
Human molecular genetics 18:2567-74 PubMed19380460
2009
Applications
ChIP
Species
Human
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