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Rabbit Monoclonal H2A crotonyl K125 antibody. Carrier free. Suitable for WB, PepArr and reacts with Mouse, Human, Synthetic peptide samples.

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Images

Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (AB284853), expandable thumbnail
  • Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (AB284853), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBPepArr
Human
Tested
Expected
Mouse
Tested
Expected
Synthetic peptide
Not recommended
Tested

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H2AFM, HIST1H2AB, H2AC8, H2AFA, HIST1H2AE, H2AC4, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AK125cr

Recommended products

Rabbit Monoclonal H2A crotonyl K125 antibody. Carrier free. Suitable for WB, PepArr and reacts with Mouse, Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP18770
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab284853 is the carrier-free version of Anti-Histone H2A (crotonyl K125) antibody [EP18770] ab195468

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (ab284853), expandable thumbnail

    Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (ab284853)

    This data was developed using Anti-Histone H2A (crotonyl K125) antibody [EP18770] ab195468, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% BSA/TBST.

    All lanes: Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (Anti-Histone H2A (crotonyl K125) antibody [EP18770] ab195468) at 1/1000 dilution

    Lane 1: Mouse embryo lysate at 10 µg

    Lane 2: Mouse testis lysate at 10 µg

    Lane 3: Human ovary lysate at 10 µg

    Lane 4: NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 10 µg

    Lane 5: HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

    Lane 6: NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate at 10 µg

    Lane 7: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

    Lane 8: Human placenta lysate at 10 µg

    Lane 9: mESC (Mouse embryonic stem cell line) whole cell lysate at 10 µg

    Lane 10: Mouse ovary lysate at 10 µg

    Lane 11: C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

    Lane 12: 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 13: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 14: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

    Exposure time: 30s

  • Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (ab284853), expandable thumbnail

    Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (ab284853)

    This data was developed using Anti-Histone H2A (crotonyl K125) antibody [EP18770] ab195468, the same antibody clone in a different buffer formulation.Anti-Histone H2A (crotonyl K125) antibody [EP18770] ab195468 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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