Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free

• WB

Unknown

Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (AB284853)

This data was developed using ab195468, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (<a href='/en-us/products/primary-antibodies/histone-h2a-crotonyl-k125-antibody-ep18770-ab195468'>ab195468</a>) at 1/1000 dilution

Lane 1:

Mouse embryo lysate at 10 µg

Lane 2:

Mouse testis lysate at 10 µg

Lane 3:

Human ovary lysate at 10 µg

Lane 4:

NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 10 µg

Lane 5:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Lane 6:

NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate at 10 µg

Lane 7:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

Lane 8:

Human placenta lysate at 10 µg

Lane 9:

mESC (Mouse embryonic stem cell line) whole cell lysate at 10 µg

Lane 10:

Mouse ovary lysate at 10 µg

Lane 11:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Lane 12:

3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 13:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 14:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 30s

• PepArr

Supplier Data

Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] - BSA and Azide free (AB284853)

This data was developed using ab195468, the same antibody clone in a different buffer formulation.ab195468 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.