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Rabbit Recombinant Monoclonal H2A hydroxy Y39 antibody. Carrier free. Suitable for WB, PepArr, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide samples.

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Images

Western blot - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (AB249961), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (AB249961), expandable thumbnail
  • Peptide Array - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (AB249961), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (AB249961), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (AB249961), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBPepArrIHC-P
Human
Tested
Expected
Tested
Mouse
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Synthetic peptide
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AC4, H2AC8, H2AFA, HIST1H2AB, HIST1H2AE, H2AFM, H2AY39y

Recommended products

Rabbit Recombinant Monoclonal H2A hydroxy Y39 antibody. Carrier free. Suitable for WB, PepArr, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide samples.

Alternative names

Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AC4, H2AC8, H2AFA, HIST1H2AB, HIST1H2AE, H2AFM, H2AY39y

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR17683

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249961 is the carrier-free version of Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961), expandable thumbnail

    Western blot - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961)

    This data was developed using Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA /TBST.

    All lanes: Western blot - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] (Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377) at 1/2000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Lane 2: NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961)

    This data was developed using Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A (hydroxyl Y39) with Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Peptide Array - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961), expandable thumbnail

    Peptide Array - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961)

    This data was developed using Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377, the same antibody clone in a different buffer formulation.

    Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961)

    This data was developed using Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H2A (hydroxyl Y39) with Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - BSA and Azide free (ab249961)

    This data was developed using Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2A (hydroxyl Y39) with Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] ab177377 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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