Rabbit Recombinant Monoclonal H2A phospho S1 antibody. Carrier free. Suitable for Dot, WB, PepArr, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples.
Images
![Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--western-blot-img39193.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959)")
![Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--dot-blot-img4350.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunocytochemistry-immunofluorescence-img14505.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959)")
![Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--peptide-array-img66654.jpg/_jcr_content/renditions/webp-475.webp "Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100983.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (AB249959)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | PepArr | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Tested |
| Rat | Tested | Expected | Expected | Expected | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AE, H2AFA, H2AC8, HIST1H2AB, H2AFM, H2AC4, H2AS1p
Recommended products
Rabbit Recombinant Monoclonal H2A phospho S1 antibody. Carrier free. Suitable for Dot, WB, PepArr, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples.
Alternative names
Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AE, H2AFA, H2AC8, HIST1H2AB, H2AFM, H2AC4, H2AS1p
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18184
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249959 is the carrier-free version of Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--western-blot-img39193.jpg "Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
All lanes: Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] (Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309) at 1/500 dilution
Lane 1: Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg
Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma), treated with 1.5μg/ml Colcemid for 12 hours, whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa, 14 kDa
Exposure time: 15s
![Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--dot-blot-img4350.jpg "Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Dot Blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Dot blot analysis of Histone H2A (phospho S1) peptide (Lane 1), unmodified Histone H2A peptide (Lane 2), Histone H4 (phospho S1) peptide (Lane 3), and unmodified Histone H4 peptide (Lane 4), labeled using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunocytochemistry-immunofluorescence-img14505.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution.![Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--peptide-array-img66654.jpg "Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Peptide Array - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100983.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on human colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71143.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97619.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat colon tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65048.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on neuron cells of human cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103626.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on mouse heart tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58199.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)
This data was developed using Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Histone H2A (phospho S1) + Histone H4 (phospho S1) with Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] ab177309 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on neuron cells of rat brain tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71143.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97619.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65048.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103626.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959), expandable thumbnail](https://content.abcam.com/products/images/histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-epr18184-bsa-and-azide-free-ab249959--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58199.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184] - BSA and Azide free (ab249959)")
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