Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] - BSA and Azide free (AB251207)

This data was developed using ab195464, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (<a href='/en-us/products/primary-antibodies/histone-h2a-symmetric-di-methyl-r11-antibody-e18766-ab195464'>ab195464</a>) at 1/500 dilution

Lane 1:

Human ovary tissue lysate at 10 µg

Lane 2:

NT2/D1(human malignant pluripotent embryonic carcinoma ) whole cell lysate at 10 µg

Lane 3:

HL-60 (Human promyelocytic leukemia cells) whole cell lysate at 10 µg

Lane 4:

NCCIT (human pluripotent embryonic carcinoma) whole cell lysate at 10 µg

Lane 5:

F9 (Mouse embryo testicular cancer cell line) whole cell lysate at 10 µg

Lane 6:

Human placenta tissue lysate at 10 µg

Lane 7:

mESC (mouse embryo stem cell) whole cell lysate at 10 µg

Lane 8:

Mouse ovary tissue lysate at 10 µg

Lane 9:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Lane 10:

3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 11:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 12:

NIH 3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 14 kDa

false

Exposure time: 3min

• PepArr

Supplier Data

Peptide Array - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] - BSA and Azide free (AB251207)

This data was developed using ab195464, the same antibody clone in a different buffer formulation.

ab195464 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.