AB240993

Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free

RabMAb
Advanced Validation
Recombinant
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Rabbit Recombinant Monoclonal H2A ubiquityl K119 antibody. Carrier free. Suitable for WB, PepArr, ICC/IF and reacts with Mouse, Rat, Human, Synthetic peptide samples.

View Alternative Names

H2AFM, HIST1H2AB, H2AC8, H2AFA, HIST1H2AE, H2AC4, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AK119ub

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Histone H2A (ubiquityl K119) with ab193203 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)

Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

PepArr

Supplier Data

Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

ab193203 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193203)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19800

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, PepArr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab240993 is the carrier-free version of ab193203.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2AC4 ubiquityl K119

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR19800] - BSA and Azide free (AB240993)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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