Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576]

• WB

Lab

Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (AB325796)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 15 kDa is unknown.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda).

Exposure time : Lane 1 : 180 seconds, lane 2 : 26 seconds

All lanes:

Western blot - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (ab325796) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) histone fraction at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) histone fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 22 kDa,15 kDa

false

• PepArr

Lab

Peptide Array - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (AB325796)

Peptide array analysis of ab325796 at (0.1 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1 : 50,000 dilution.

ab325796 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (AB325796)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH/3T3 (Mouse embryonic fibroblast) cells and 5 g of ab325796 [EPR28331-576]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (AB325796)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervical adenocarcinoma epithelial cell) cells and 5 g of ab325796 [EPR28331-576]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Histone H2A (ubiquityl K119) antibody [EPR28331-576] (AB325796)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervical adenocarcinoma epithelial cell) cells and 5 g of ab325796 [EPR28331-576]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.