Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody that is used to detect Histone H2A.X in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 150 publications
- Trusted since 2004
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia-Reperfusion-Induced Acute Kidney Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJelena Nesovic Ostojic,Maja Zivotic,Sanjin Kovacevic,Milan Ivanov,Predrag Brkic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Una Jovana Vajic,Zoran Miloradovic,Djurdjica Jovovic,Sanja Radojevic SkodricPubMed 37175793
- Oncology reports 49:2023AZD6738 promotes the tumor suppressive effects of trifluridine in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinnosuke Harata,Takuya Suzuki,Hiroki Takahashi,Takahisa Hirokawa,Akira Kato,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Shuji TakiguchiPubMed 36734271
- Molecular and cellular biology 42:e00379222022Impact of Nuclear NAD Synthesis via Histone Dynamics on DNA Repair during Cellular Senescence To Prevent Tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasae Ikura,Kanji Furuya,Tomonari Matsuda,Tsuyoshi IkuraPubMed 36278823
- Scientific reports 12:177292022Noxa and Mcl-1 expression influence the sensitivity to BH3-mimetics that target Bcl-xL in patient-derived glioma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Belén Vera,Olivia Morris-Hanon,Germán Ignacio Nogueiras,Luisina Belén Ripari,Myrian Inés Esquivel,Carolina Perez-Castro,Leonardo Romorini,Gustavo Emilio Sevlever,María Elida Scassa,Guillermo Agustín Videla-RichardsonPubMed 36273072
- Molecular cell 82:3794-3809.e82022Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlse Delint-Ramirez,Lahiri Konada,Lance Heady,Richard Rueda,Alvaro Sebastian Vaca Jacome,Eric Marlin,Charlotte Marchioni,Amir Segev,Oleg Kritskiy,Satoko Yamakawa,Andrew H Reiter,Li-Huei Tsai,Ram MadabhushiPubMed 36206766
- Journal of medical genetics 59:1227-12332022Loss of seryl-tRNA synthetase () causes complex spastic paraplegia and cellular senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdgard Verdura,Bruno Senger,Miquel Raspall-Chaure,Agatha Schlüter,Nathalie Launay,Montserrat Ruiz,Carlos Casasnovas,Agustí Rodriguez-Palmero,Alfons Macaya,Hubert Dominique Becker,Aurora PujolPubMed 36041817
- iScience 25:1049442022Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeilei Qi,Reka Chakravarthy,Monica M Li,Chu-Xia Deng,Rong Li,Yanfen HuPubMed 36065181
- Life science alliance 5:2022RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRie Kanao,Hidehiko Kawai,Toshiyasu Taniguchi,Minoru Takata,Chikahide MasutaniPubMed 35905994
- Oncology reports 47:2022ATR inhibitor AZD6738 increases the sensitivity of colorectal cancer cells to 5‑fluorouracil by inhibiting repair of DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuya Suzuki,Takahisa Hirokawa,Anri Maeda,Shinnosuke Harata,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Nozomi Nakai,Yuzo Maeda,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Hiroki Takahashi,Shuji TakiguchiPubMed 35191521
- Protein & cell 13:580-6012022BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingzhu Wang,Kun Zhao,Meng Liu,Mengting Wang,Zhibin Qiao,Shanru Yi,Yonghua Jiang,Xiaochen Kou,Yanhong Zhao,Jiqing Yin,Tianming Li,Hong Wang,Cizhong Jiang,Shaorong Gao,Jiayu ChenPubMed 35147915
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Gorilla | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1 µg/mL | Notes - |
Species Mouse | Dilution info 0.1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Rhesus monkey, Gorilla | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X
Recommended products
Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody that is used to detect Histone H2A.X in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 150 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The phosphorylated form of this Ab is known as gamma H2A.X, when phosphorylated at Ser 139.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Histone H2A.X antibody (ab11175)
All lanes: Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL
All lanes: HEK293T cells at 50 µg
Predicted band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (ab11175)
mmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling H2A.X with ab11175 at 1 μg/mL.
Western blot - Anti-Histone H2A.X antibody (ab11175)
Detection: Chemiluminescence
All lanes: Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL
Lane 1: F9 cell lysate (prepared using RIPA lysis buffer) at 50 µg
Lane 2: L1210 cell lysate (prepared using RIPA lysis buffer) at 50 µg
Predicted band size: 15 kDa
Exposure time: 30s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com