AB11175

Anti-Histone H2A.X antibody

Name: Anti-Histone H2A.X antibody (ab11175) | Abcam
Brand: Abcam Limited
SKU: AB11175
Price: 450 USD
Availability: InStock
Rating: 5 (43 reviews)

(43 Reviews)

(182 Publications)

Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody detecting Histone H2A.X in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 150 publications
- Trusted since 2004

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

3 Images

Supplier Data

Western blot - Anti-Histone H2A.X antibody (AB11175)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

Lane 1:

F9 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Lane 2:

L1210 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Predicted band size: 15 kDa

false

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

mmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling H2A.X with ab11175 at 1 μg/mL.

Unknown

Western blot - Anti-Histone H2A.X antibody (AB11175)

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

All lanes:

HEK293T cells at 50 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

Trusted by the scientific community
Anti-Histone H2A.X (ab11175) was first used in a scientific publication in 2004 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H2A.X (ab11175) has over 40 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H2AX

style

left-column

Publications (182)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 18:46 PubMed40665417

2025

H2A.X N-terminal acetylation is a newly identified NAA40-mediated modification that is responsive to UV irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Klavaris,Costas Koufaris,Roberta Noberini,Maria Kouma,Christina Demetriadou,Alessandro Ghiringhelli,Nikolas Dietis,Tiziana Bonaldi,Antonis Kirmizis

Genes to cells : devoted to molecular & cellular mechanisms 30:e70031 PubMed40452456

2025

PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yurina Abe,Hayaki Ikegame,Yuina Tsuchiya,Ryotaro Nishi

Oncogene 44:1375-1386 PubMed39994376

2025

Cyclin E1 overexpression sensitizes ovarian cancer cells to WEE1 and PLK1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xi,Akiko Kunita,Miho Ogawa,Mirei Ka,Saki Tanimoto,Saki Tsuchimochi,Saeko Nagai,Asami Matsunaga,Tomohiko Fukuda,Kousuke Watanabe,Kenbun Sone,Aya Shinozaki-Ushiku,Kei Kawana,Tetsuo Ushiku,Yutaka Osuga,Kazuhiro Katayama,Hidenori Kage,Katsutoshi Oda

Nature communications 15:9171 PubMed39448645

2024

Epigenetic modulation via the C-terminal tail of H2A.Z.

Applications

Unspecified application

Species

Unspecified reactive species

László Imre,Péter Nánási,Ibtissem Benhamza,Kata Nóra Enyedi,Gábor Mocsár,Rosevalentine Bosire,Éva Hegedüs,Erfaneh Firouzi Niaki,Ágota Csóti,Zsuzsanna Darula,Éva Csősz,Szilárd Póliska,Beáta Scholtz,Gábor Mező,Zsolt Bacsó,H T Marc Timmers,Masayuki Kusakabe,Margit Balázs,György Vámosi,Juan Ausio,Peter Cheung,Katalin Tóth,David Tremethick,Masahiko Harata,Gábor Szabó

European thyroid journal 13: PubMed39047147

2024

A post-irradiation-induced replication stress promotes RET proto-oncogene breakage.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Hecht,Laura Valerio,Carlos Frederico Lima Gonçalves,Marylin Harinquet,Rabii Ameziane El Hassani,Denise P Carvalho,Stephane Koundrioukoff,Jean-Charles Cadoret,Corinne Dupuy

International journal of molecular medicine 53: PubMed38695243

2024

Predictive DNA damage signaling for low‑dose ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-In Park,Seung-Youn Jung,Kyung-Hee Song,Dong-Hyeon Lee,Jiyeon Ahn,Sang-Gu Hwang,In-Su Jung,Dae-Seog Lim,Jie-Young Song

Biology of reproduction 111:483-495 PubMed38625059

2024

Trajectory of primordial follicle depletion is accelerated in obese mice in response to 7,12-dimethylbenz[a]anthracene exposure†.

Applications

Unspecified application

Species

Unspecified reactive species

Jaspreet K Rishi,Kelsey Timme,Hunter E White,Karl C Kerns,Aileen F Keating

Journal of radiation research 65:263-271 PubMed38461549

2024

Inhibition of intracellular ATP synthesis impairs the recruitment of homologous recombination factors after ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Hayashi,Hikaru Okumura,Mayu Isono,Motohiro Yamauchi,Daiki Unami,Rahmartani Tania Lusi,Masamichi Yamamoto,Yu Kato,Yuki Uchihara,Atsushi Shibata

The Journal of biological chemistry 300:105538 PubMed38072046

2023

Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.

Applications

Unspecified application

Species

Unspecified reactive species

Peijun Wang,Na Fan,Wanting Yang,Pengbo Cao,Guojun Liu,Qi Zhao,Pengfei Guo,Xihe Li,Xinhua Lin,Ning Jiang,Buhe Nashun

Journal of thoracic disease 15:3350-3358 PubMed37426130

2023

Overexpressed collaborates with MMB to increase inhibitor sensitivity in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tan,Jingbo Ma,Weiqiang Zhang,Jing Zhao,Keqiang Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A.X antibody

Western blot - Anti-Histone H2A.X antibody (AB11175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

Western blot - Anti-Histone H2A.X antibody (AB11175)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (182)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise