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AB140498

Anti-Histone H2A.X antibody

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(4 Publications)

Goat Polyclonal H2A.X antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Histone H2A.X with ab140498 at 1/5000 (0.2µg/ml). Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB140498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling Histone H2A.X with ab140498 at 1/1000 (1µg/ml). Detection : DAB.

Western blot - Anti-Histone H2A.X antibody (AB140498)
  • WB

Unknown

Western blot - Anti-Histone H2A.X antibody (AB140498)

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab140498) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Predicted band size: 15 kDa

true

Exposure time: 30s

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab140498 was affinity purified using an epitope specific to Histone H2A.X immobilized on a solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H2AX

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6228 PubMed36266274

2022

A BDNF-TrkB autocrine loop enhances senescent cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Anerillas,Allison B Herman,Rachel Munk,Amanda Garrido,Kwan-Wood Gabriel Lam,Matthew J Payea,Martina Rossi,Dimitrios Tsitsipatis,Jennifer L Martindale,Yulan Piao,Krystyna Mazan-Mamczarz,Jinshui Fan,Chang-Yi Cui,Supriyo De,Kotb Abdelmohsen,Rafael de Cabo,Myriam Gorospe

Cells 8: PubMed31752383

2019

Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Hong,Jinke Li,Siying Li,Mohammed M Almutairi

Developmental cell 50:509-524.e10 PubMed31327739

2019

PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Sandra Jones,Adi Minis,Jose Rodriguez,Henrik Molina,Hermann Steller

Cancer research 79:819-829 PubMed30573522

2018

EGFR and c-MET Cooperate to Enhance Resistance to PARP Inhibitors in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongzhu Dong,Yi Du,Hui Li,Chunxiao Liu,Yongkun Wei,Mei-Kuang Chen,Xixi Zhao,Yu-Yi Chu,Yufan Qiu,Lunxiu Qin,Hirohito Yamaguchi,Mien-Chie Hung
View all publications

Product promise

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