AB232908

Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody

Name: Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (ab232908) | Abcam
Brand: Abcam Limited
SKU: AB232908
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal H2A.Z acetyl K7 + K7 antibody. Suitable for ChIP, Dot, ICC/IF, ChIP-seq and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H2AZ1 acetyl K7 + K11 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

H2AFZ, H2AZ, H2AZ1, Histone H2A.Z, H2A/z

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for H2A.Z (acetyl K4 + K7 + K11) (green) using ab232908 at 1/500 dilution in ICC/IF. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with ab232908 (left) at 1/500 dilution in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

ChIP-sequencing - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP-seq

Supplier Data

ChIP-sequencing - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP was performed with 1 μg of ab232908 against H2A.Z (acetyl K4 + K7 + K11) on sheared chromatin from 1 million HeLa S3 cells. IgG (2 μg/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows shows the peak distribution along the complete sequence. These results clearly show an enrichment of the H2A.Z acetylation at the promoters of active genes.

ChIP - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP

Supplier Data

ChIP - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP assays were performed using HeLa S3 cells, ab232908 against H2A.Z (acetyl K4 + K7 + K11) and optimized primer pairs for qPCR. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/ IP) was used as negative IP control. QPCR was performed using primers specific for the promoters of the ACTB and EIF4A2 genes, used as positive control targets and for the coding region of the MYT1 gene, used as a negative control target. The figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H2A.Z is present at active promoters.

ChIP-seq

Supplier Data

ChIP-sequencing - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP was performed with 1 μg of ab232908 against H2A.Z (acetyl K4 + K7 + K11) on sheared chromatin from 1 million HeLa S3 cells. IgG (2 μg/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the peak distribution along 600 kb region of the X-chromosome and in 100 kb regions surrounding the EIF4A2, ACTB and GAPDH genes. These results clearly show an enrichment of the H2A.Z acetylation at the promoters of active genes.

Supplier Data

Dot Blot - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

Dot Blot analysis was performed to test the cross reactivity of ab232908 against H2A.Z (acetyl K4 + K7 + K11) with peptides containing other histone acetylations and the unmodified H2A.Z sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at 1/20,000 dilution. ab232908 shows a high specificity for the modification of interest.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP-seq, ICC/IF, Dot, ChIP

applications

Immunogen

Synthetic Peptide within Human H2AZ1 acetyl K7 + K11 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/20000", "Dot-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "1 µg/cells for 6 µg/cells", "ChIPseq-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.05% Sodium azide, 0.05% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

See full target information H2AZ1 acetyl K7 + K7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:322 PubMed40598498

2025

The Drosophila histone variant H2Av facilitates Notch signaling activity in a two-tier regulatory fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Chen,Xinyue Zhang,Yu Song,Jie Shen,Junzheng Zhang

Cell death & disease 12:609 PubMed34120148

2021

H2A.Z acetylation by lincZNF337-AS1 via KAT5 implicated in the transcriptional misregulation in cancer signaling pathway in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Yuan,Wen Cao,Hongbing Zhou,Haixin Qian,Honggang Wang

Molecular therapy. Methods & clinical development 21:530-547 PubMed33997102

2021

Genetically blocking CRISPR-Cas9 protects against lethal liver injury in a pig model of tyrosinemia type I.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Gu,Qin Yang,Bangzhu Chen,Ya-Nan Bie,Wen Liu,Yuguang Tian,Hongquan Luo,Tao Xu,Chunjin Liang,Xing Ye,Yan Liu,Xiangwu Tang,Weiwang Gu

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP-sequencing - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

ChIP-sequencing - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

Dot Blot - Anti-Histone H2A.Z (acetyl K4 + K7 + K11) antibody (AB232908)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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