Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) is a rabbit polyclonal antibody that is used to detect Histone H2A.Z in Western Blot, IP, ChIP. Suitable for Cow, Human, Mouse, Rat samples.
- Over 140 publications
- Trusted since 2004
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
ChIP | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Not recommended |
Mouse | Expected | Tested | Not recommended |
Rat | Expected | Tested | Not recommended |
Arabidopsis thaliana | Predicted | Predicted | Not recommended |
Cow | Expected | Tested | Not recommended |
Sheep | Predicted | Predicted | Not recommended |
Xenopus laevis | Predicted | Predicted | Not recommended |
Zebrafish | Predicted | Predicted | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes Abcam recommends using BSA as the blocking agent. |
Species Human | Dilution info 1/1000 | Notes Abcam recommends using BSA as the blocking agent. |
Species Mouse | Dilution info 1/1000 | Notes Abcam recommends using BSA as the blocking agent. |
Species Cow | Dilution info 1/1000 | Notes Abcam recommends using BSA as the blocking agent. |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat, Cow, Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish | Dilution info - | Notes - |
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Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
H2AFZ, H2AZ, H2AZ1, Histone H2A.Z, H2A/z
Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) is a rabbit polyclonal antibody that is used to detect Histone H2A.Z in Western Blot, IP, ChIP. Suitable for Cow, Human, Mouse, Rat samples.
- Over 140 publications
- Trusted since 2004
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1 µg/mL
Lane 1: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 4: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 16 kDa, 36 kDa
Exposure time: 3min
Chromation from mouse bone marrow derived macrophages. ChIP experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue, (ab4174 at 4μg), H3K4me1 (green, Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895 at 1 μg), H3K4me3 (yellow, Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade ab8580 at 1 μg) and H3K27ac (red, Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 at 1 μg). For these experiments cross-linked chromatin was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Histone H2A.Z Western blot staining of Calf thymus histone lysate using rabbit Anti-Histone H2A.Z antibody
All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/500 dilution
All lanes: Calf thymus histone lysate
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 15 kDa
Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol.
Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab4174 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Blocked with 3% BSA for 1 hour at 20°C.
Primary incubation in TBS tween + 3% BSA at 20°C for 1 hour.
All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/1000 dilution
Lane 1: Native recombinant octamers K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells at 3 µg
Lane 2: Native recombinant octamers K562 cells at 1.5 µg
Lane 3: Recombinant Human octamers containing H2A at 1 µg
Lane 4: Recombinant Human octamers containing H2A at 0.5 µg
Lane 5: Recombinant Human octamers containing H2A.Z.2.1 at 0.5 µg
Lane 6: Recombinant Human octamers containing H2A.Z.1 at 0.5 µg
All lanes: HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 5min
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