Skip to main content

Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) is a rabbit polyclonal antibody that is used to detect Histone H2A.Z in Western Blot, IP, ChIP. Suitable for Cow, Human, Mouse, Rat samples.



- Over 140 publications

- Trusted since 2004


Images

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174), expandable thumbnail
  • ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174), expandable thumbnail
  • Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174), expandable thumbnail
  • ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174), expandable thumbnail
  • Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Rat
Expected
Tested
Not recommended
Arabidopsis thaliana
Predicted
Predicted
Not recommended
Cow
Expected
Tested
Not recommended
Sheep
Predicted
Predicted
Not recommended
Xenopus laevis
Predicted
Predicted
Not recommended
Zebrafish
Predicted
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Cow, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1 µg/mL
Notes

Abcam recommends using BSA as the blocking agent.

Species
Human
Dilution info
1/1000
Notes

Abcam recommends using BSA as the blocking agent.

Species
Mouse
Dilution info
1/1000
Notes

Abcam recommends using BSA as the blocking agent.

Species
Cow
Dilution info
1/1000
Notes

Abcam recommends using BSA as the blocking agent.

Predicted
Predicted

Species
Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Cow, Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Alternative names

Recommended products

Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) is a rabbit polyclonal antibody that is used to detect Histone H2A.Z in Western Blot, IP, ChIP. Suitable for Cow, Human, Mouse, Rat samples.



- Over 140 publications

- Trusted since 2004

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Cow, Human, Mouse, Rat samples.

What is the molecular weight of Histone H2A.Z?


Anti-Histone H2A.Z - ChIP Grade (ab4174) specifically detects a band for Histone H2A.Z (UniProt: P0C0S5) at a molecular weight of 13.4kDa.

Trusted by the scientific community


Anti-Histone H2A.Z - ChIP Grade (ab4174) was first used in a scientific publication in 2004 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists


Anti-Histone H2A.Z - ChIP Grade (ab4174) has over 25 independent reviews from customers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174), expandable thumbnail

    Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1 µg/mL

    Lane 1: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 4: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 16 kDa, 36 kDa

    Exposure time: 3min

  • ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174), expandable thumbnail
    Gjidoda et al PLoS One. 2014 Apr 4;9(4):e93971. doi: 10.1371/journal.pone.0093971. eCollection 2014. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    Changes in nucleosome occupancy upon LPS induction at a putative distal enhancer and promoter of IL1A, kinetics of nucleosome removal, and changes in histone modifications.

    Chromation from mouse bone marrow derived macrophages. ChIP experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue, (ab4174 at 4μg), H3K4me1 (green, Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895 at 1 μg), H3K4me3 (yellow, Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade ab8580 at 1 μg) and H3K27ac (red, Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 at 1 μg). For these experiments cross-linked chromatin was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).

  • Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174), expandable thumbnail

    Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    Histone H2A.Z Western blot staining of Calf thymus histone lysate using rabbit Anti-Histone H2A.Z antibody

    All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/500 dilution

    All lanes: Calf thymus histone lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 15 kDa

  • ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174), expandable thumbnail

    ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol.

    Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab4174 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174), expandable thumbnail
    This image is courtesy of a customer review submitted by Ragnhild Eskeland

    Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    Blocked with 3% BSA for 1 hour at 20°C.

    Primary incubation in TBS tween + 3% BSA at 20°C for 1 hour.

    All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/1000 dilution

    Lane 1: Native recombinant octamers K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells at 3 µg

    Lane 2: Native recombinant octamers K562 cells at 1.5 µg

    Lane 3: Recombinant Human octamers containing H2A at 1 µg

    Lane 4: Recombinant Human octamers containing H2A at 0.5 µg

    Lane 5: Recombinant Human octamers containing H2A.Z.2.1 at 0.5 µg

    Lane 6: Recombinant Human octamers containing H2A.Z.1 at 0.5 µg

    Secondary

    All lanes: HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 15 kDa

    Exposure time: 5min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com