Anti-Histone H2A.Z antibody - ChIP Grade

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

ab97966 staining Histone H2A.Z in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab97966 at 0.5μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H2A.Z staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

IHC image of ab97966 staining Histone H3 (phospho T45) in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97966, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IP

Supplier Data

Immunoprecipitation - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

ab97966 immunoprecipitating Histone H2A.Z protein in HeLa whole cell lysate/extract. Lane 1 : 50 μg HeLa whole cell lysate/extract. Lane 2 : Control with 2 μg of preimmune rabbit IgG. Lane 3 : Immunoprecipitation of Histone H2A.Z protein by 2 μg of ab97966. The immunoprecipitated Histone H2A.Z protein was detected with ab97966 diluted at 1 : 1000. Anti-rabbit IgG was used as a secondary antibody.

All lanes:

Immunoprecipitation - Anti-Histone H2A.Z antibody - ChIP Grade (ab97966)

Predicted band size: 14 kDa

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• ChIP

Lab

ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab97966 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

• ChIP

Supplier Data

ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein DNA in ChIP experiments.
ChIP Sample : HeLa whole cell lysate/extract
A. 5 µg preimmune rabbit IgG
B. 5 µg of ab97966
The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Immunohistochemical analysis of paraffin embedded mouse fore brain tissue stained for Histone H2A.Z protein at nucleus with ab97966 at a 1/1000 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• WB

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Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

12% SDS PAGE

All lanes:

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab97966) at 1/3000 dilution

Lane 1:

MOLT4 whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 14 kDa

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• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Immunofluorescence analysis of MDCK cells fixed in 4% paraformaldehyde at RT for 15 min labelling Histone H2A.Z protein at nucleus using ab97966 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red). Scale bar = 10 µm

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Immunohistochemical analysis of paraffin embedded cat spleen tissue stained for Histone H2A.Z protein at nucleus with ab97966 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Immunohistochemical analysis of paraffin embedded cat kidney tissue stained for Histone H2A.Z protein at nucleus with ab97966 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab97966) at 1/3000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 14 kDa

false

• WB

Supplier Data

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB97966)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab97966) at 1/3000 dilution

All lanes:

PG 4 Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 14 kDa

false