AB124793

Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade

Name: Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (ab124793) | Abcam
Brand: Abcam Limited
SKU: AB124793
Price: 580 USD
Availability: InStock
Rating: 5 (6 reviews)

RabMAb
Advanced Validation
Recombinant
What is this?

(6 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal H2A.Z antibody. Suitable for IHC-P, WB, ChIP-seq and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

H2AFZ, H2AZ, H2AZ1, Histone H2A.Z, H2A/z

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

ab124793 at a 1/250 dilution staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ChIP-sequencing - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

ChIP-seq

Lab

ChIP-sequencing - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 μg of ab124793 [EPR6172]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. Additional screenshots of mapped reads can be downloaded here.

Unknown

Western blot - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

All lanes:

Western blot - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (ab124793) at 1/1000 dilution

Lane 1:

C6 lysates at 10 µg

Lane 2:

Raw 264.7 lysates at 10 µg

Lane 3:

PC-12 lysates at 10 µg

Lane 4:

NIH/3T3 lysates at 10 µg

Lane 5:

HeLa lysates at 10 µg

Lane 6:

HepG2 lysates at 10 µg

Lane 7:

Human thymus lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 14 kDa

false

Carrier free

Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6172

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ChIP-seq, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).

The immunogen used for this product (H2A.Z/H2AZ1) shares 100% homology with H2A.V/H2AZ2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "antigen retrieval is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "4 µg/cells for 7 µg/cells", "ChIPseq-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

See full target information H2AZ1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 23:1735-1748 PubMed36456734

2022

Fungal sensing by dectin-1 directs the non-pathogenic polarization of T17 cells through balanced type I IFN responses in human DCs.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja I Gringhuis,Tanja M Kaptein,Ester B M Remmerswaal,Agata Drewniak,Brigitte A Wevers,Bart Theelen,Geert R A M D'Haens,Teun Boekhout,Teunis B H Geijtenbeek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

ChIP-sequencing - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

Western blot - Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (AB124793)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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