Anti-Histone H2B (acetyl K11) antibody

• ICC

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Immunocytochemistry - Anti-Histone H2B (acetyl K11) antibody (AB240613)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H2B (acetyl K11) using ab240613 at 1/20 dilution in ICC.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight.

The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ChIP

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ChIP - Anti-Histone H2B (acetyl K11) antibody (AB240613)

Hela (human epithelial cell line from cervix adenocarcinoma; 10⁶, treated with 30 mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240613 or a control normal rabbit IgG.

The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

• WB

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Western blot - Anti-Histone H2B (acetyl K11) antibody (AB240613)

All lanes:

Western blot - Anti-Histone H2B (acetyl K11) antibody (ab240613) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

Lane 4:

HEK-293 whole cell lysate, untreated (-)

Lane 5:

A549 whole cell lysate, untreated (-)

Lane 6:

K562 whole cell lysate, untreated (-)

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 14 kDa

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