Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Expected | Tested |
Rat | Predicted | Predicted |
African green monkey | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Select an associated product type
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
H2BFT, HIRIP1, HIST1H2BK, H2BC12, HIST1H2BK, HIRIP1, H2BFT, Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, H2BK12ac
Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
ab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Blue Ice
+4°C
-20°C
Stable for 12 months at -20°C
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
All lanes: Western blot - Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution
Lane 1: COS-7 (African green monkey kidney fibroblast-like cell line) cells treated with TSA (400nM, 24hours), without acetylated peptide
Lane 2: COS-7 (African green monkey kidney fibroblast-like cell line) cells treated with TSA (400nM, 24hours)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com