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Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody (AB61228), expandable thumbnail
  • Western blot - Anti-Histone H2B (acetyl K12) antibody (AB61228), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7. The exact immunogen used to generate this antibody is proprietary information. Database link O60814

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested
Rat
Predicted
Predicted
African green monkey
Tested
Expected

Tested
Tested

Species

African green monkey

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/100.00000

Notes

-

Expected
Expected

Species

African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7. The exact immunogen used to generate this antibody is proprietary information. Database link O60814
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab61228 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody (ab61228), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody (ab61228)

    Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H2B (acetyl K12) antibody (ab61228) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.

  • Western blot - Anti-Histone H2B (acetyl K12) antibody (ab61228), expandable thumbnail

    Western blot - Anti-Histone H2B (acetyl K12) antibody (ab61228)

    All lanes: Western blot - Anti-Histone H2B (acetyl K12) antibody (ab61228) at 1/500 dilution

    Lane 1: COS-7 (African green monkey kidney fibroblast-like cell line) cells treated with TSA (400nM, 24hours), without acetylated peptide

    Lane 2: COS-7 (African green monkey kidney fibroblast-like cell line) cells treated with TSA (400nM, 24hours)

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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