Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC18 acetyl K12 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
ChIP | Dot | WB | ICC/IF | ChIP-seq | |
---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Tested |
Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes 0.5 - 5 μg per ChIP |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes 0.5 - 5 μg per ChIP |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
HIST2H2BF, HIST2H2BF, H2BC18, Histone H2B type 2-F, H2B-clustered histone 18, H2BK12ac
Rabbit Polyclonal H2B acetyl K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC18 acetyl K12 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
Affinity purification Protein A
Due to sequence homology we expect this antibody to detect K12 acetylation on multiple H2B variants.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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ChIP analyis using Human HeLa cells, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls, respectively. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP-seq analysis using Human HeLaS3 cells, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 0.5 μg and optimized PCR primer sets for qPCR as described above. The 51 bp tags were aligned to the Human genome using the BWA algorithm. The figure shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.
Dot Blot analysis of peptides containing the unmodified H2B and other histone modifications, labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 1/5000 dilution. 0.2-100 pmol of the peptide containing the respective modifications were spotted onto the membrane.
All lanes: Western blot - Anti-Histone H2B (acetyl K12) antibody - ChIP Grade (ab195494) at 1/1000 dilution
Lane 1: HeLa whole cell extracts at 25 µg
Lane 2: HeLa histone extracts at 15 µg
Lane 3: Recombinant H2A at 1 µg
Lane 4: Recombinant H2B at 1 µg
Lane 5: Recombinant H3 at 1 µg
Lane 6: Recombinant H4 at 1 µg
Predicted band size: 14 kDa
Immunofluorescent analysis of HeLa cells labeling Histone H2B type 1-C/E/F/G/I (acetyl K12) with ab195494 at 1/500 dilution in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488 (left), DAPI (center) or merged (right). Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA.
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