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Rabbit Polyclonal H2B acetyl K15 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H2BC18 acetyl K15 conjugated to Keyhole Limpet Haemocyanin.

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Images

ChIP - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (AB195652), expandable thumbnail
  • ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (AB195652), expandable thumbnail
  • Dot Blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (AB195652), expandable thumbnail
  • Western blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (AB195652), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (AB195652), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human H2BC18 acetyl K15 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q5QNW6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPDotWBICC/IFChIP-seq
Human
Tested
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 0.5-5 μg of antibody per 1.5x106 cells.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 2 μg of antibody per 1.5x106 cells.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Polyclonal H2B acetyl K15 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H2BC18 acetyl K15 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human H2BC18 acetyl K15 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q5QNW6
Purification technique

Affinity purification

Specificity

Due to sequence homology we expect this antibody to detect K15 acetylation on multiple H2B variants.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652), expandable thumbnail

    ChIP - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)

    CHIP analysis of Histone H2B type 1-C/E/F/G/I (acetyl K15) in HeLa cells using ab195652 at 2 μg/IP, and optimized PCR primer sets for qPCR. ChIP was performed with the kit on sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652), expandable thumbnail

    ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)

    ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 2 μg of ab195652, as described for the CHIP application. The IP'd DNA was subsequently analysed on a sequencer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the Human genome using the BWA algorithm. This image shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (sections A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (sections C and D). The position of the amplicon used for ChIPqPCR is indicated by an arrow.

  • Dot Blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652), expandable thumbnail

    Dot Blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)

    To test the cross reactivity of ab195652, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified Histone H2B type 1-C/E/F/G/I. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1/20,000. This image shows a high specificity of the antibody for the modification of interest.

  • Western blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652), expandable thumbnail

    Western blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)

    ab195652 was diluted in TBS-Tween containing 5% skimmed milk.

    All lanes: Western blot - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652) at 1/500 dilution

    Lane 1: whole extract from Hela cell at 25 µg

    Lane 2: histone extract from Hela cell at 15 µg

    Lane 3: recombinant histone H2A at 1 µg

    Lane 4: recombinant histone H2B at 1 µg

    Lane 5: recombinant histone H3 at 1 µg

    Lane 6: recombinant histone H4 at 1 µg

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody - ChIP Grade, purified (ab195652)

    Immunofluorescent analysis of Histone H2B type 1-C/E/F/G/I (acetyl K15) in HeLa cells using ab195652 at a 1/500 dilution in blocking solution, followed by an anti-rabbit antibody conjugated to Alexa488 (left). The cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

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Product protocols

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