AB62335

Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade

Name: Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335) | Abcam
Brand: Abcam Limited
SKU: AB62335
Price: 340 USD
Availability: InStock
Rating: 4 (6 reviews)

RabMAb
Advanced Validation
Recombinant
What is this?

(6 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal H2B acetyl K15 antibody. Suitable for PepArr, WB, IHC-P, ChIP-seq and reacts with Synthetic peptide, Human, Mouse samples. Cited in 5 publications.

View Alternative Names

HIST2H2BF, H2BC18, Histone H2B type 2-F, H2B-clustered histone 18, H2BK15ac, Hist1h2bm, H2bc14, Histone H2B type 1-M, H2B 291B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.

ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

ChIP-seq

Lab

ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 μg of ab62335 [EP955Y]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. Additional screenshots of mapped reads can be downloaded here.

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Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

The bottom image shows beta Tubulin as a positive control.

All lanes:

Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335) at 1/2000 dilution

Lane 1:

HeLa cell lysate (untreated) at 10 µg

Lane 2:

HeLa cell lysate (treated with TSA) at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

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Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

PepArr

Lab

Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

ab62335 at 0.5 μg/mL was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). A Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution was used as the secondary antibody. Washing buffer and time was protein Array Washing Buffer, 2*10mins and 2*5mins and blocking buffer was 5% BSA in TBST. Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

Scanner details : Innoscan 710

Carrier free

Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP955Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, PepArr, WB, ChIP-seq

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects Histone H2B acetylated on Lysine 15.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "4 µg/cells for 7 µg/cells", "ChIPseq-species-notes": "" }, "Mouse": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "4 µg for 10^7 Cells", "ChIPseq-species-notes": "" }, "Rat": { "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Synthetic peptide": { "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "0.5 µg/mL", "PepArr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2BC18 acK15

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4103 PubMed37460559

2023

Epigenetic mechanisms to propagate histone acetylation by p300/CBP.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Kikuchi,Satoshi Morita,Masatoshi Wakamori,Shin Sato,Tomomi Uchikubo-Kamo,Takehiro Suzuki,Naoshi Dohmae,Mikako Shirouzu,Takashi Umehara

Frontiers in immunology 12:779667 PubMed35046941

2022

Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xia,Yu Liu,Zhiwei Zhang,Yajuan Gong,Tianyi Yu,Dan Zhao,Wen Qiu,Yingwei Wang,Jing Zhang

Cell metabolism 29:886-900.e5 PubMed30661930

2019

ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Lu,Wen-Wei Zhu,Xuan Wang,Jing-Jie Tang,Kai-Li Zhang,Guang-Yang Yu,Wei-Qing Shao,Zhi-Fei Lin,Sheng-Hao Wang,Lu Lu,Jian Zhou,Lian-Xin Wang,Hu-Liang Jia,Qiong-Zhu Dong,Jin-Hong Chen,Jian-Quan Lu,Lun-Xiu Qin

Scientific reports 4:5432 PubMed24961246

2014

Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Dong,Haojie Hao,Lei Xia,Jiejie Liu,Dongdong Ti,Chuan Tong,Qian Hou,Qingwang Han,Yali Zhao,Huiling Liu,Xiaobing Fu,Weidong Han

Experimental and molecular pathology 88:67-76 PubMed19883641

2009

Epigenetic modifications induced by RGC-32 in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia I Vlaicu,Cosmin A Tegla,Cornelia D Cudrici,Matthew Fosbrink,Vingh Nguyen,Philippe Azimzadeh,Violeta Rus,Hegang Chen,Petru A Mircea,Abulkalam Shamsuddin,Horea Rus

View all publications

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peptideArrayWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Properties and storage information

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Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise