Rabbit Recombinant Monoclonal H2B acetyl K15 antibody. Suitable for PepArr, WB, IHC-P, ChIP-seq and reacts with , Human, Mouse samples. Cited in 5 publications.
Images
![ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--chip-sequencing-img175997.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)")
![Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--western-blot-img6802.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135392.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)")
![Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--peptide-array-img436302.jpg/_jcr_content/renditions/webp-475.webp "Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (AB62335)")
Publications
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- Nature communications 14:41032023Epigenetic mechanisms to propagate histone acetylation by p300/CBP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaki Kikuchi,Satoshi Morita,Masatoshi Wakamori,Shin Sato,Tomomi Uchikubo-Kamo,Takehiro Suzuki,Naoshi Dohmae,Mikako Shirouzu,Takashi UmeharaPubMed 37460559
- Frontiers in immunology 12:7796672022Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Xia,Yu Liu,Zhiwei Zhang,Yajuan Gong,Tianyi Yu,Dan Zhao,Wen Qiu,Yingwei Wang,Jing ZhangPubMed 35046941
- Cell metabolism 29:886-900.e52019ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Lu,Wen-Wei Zhu,Xuan Wang,Jing-Jie Tang,Kai-Li Zhang,Guang-Yang Yu,Wei-Qing Shao,Zhi-Fei Lin,Sheng-Hao Wang,Lu Lu,Jian Zhou,Lian-Xin Wang,Hu-Liang Jia,Qiong-Zhu Dong,Jin-Hong Chen,Jian-Quan Lu,Lun-Xiu QinPubMed 30661930
- Scientific reports 4:54322014Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Dong et. al.PubMed 24961246
- Experimental and molecular pathology 88:67-762009Epigenetic modifications induced by RGC-32 in colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia I Vlaicu,Cosmin A Tegla,Cornelia D Cudrici,Matthew Fosbrink,Vingh Nguyen,Philippe Azimzadeh,Violeta Rus,Hegang Chen,Petru A Mircea,Abulkalam Shamsuddin,Horea RusPubMed 19883641
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| PepArr | IP | WB | IHC-P | ChIP-seq | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested | Tested |
| Mouse | Predicted | Not recommended | Expected | Expected | Expected |
| Rat | Predicted | Not recommended | Predicted | Predicted | Predicted |
PepArr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/cells for 7 µg/cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 4 µg for 107 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Targets
H2bc14
Alternative names
HIST2H2BF, H2BC18, Histone H2B type 2-F, H2B-clustered histone 18, H2BK15ac
Recommended products
Rabbit Recombinant Monoclonal H2B acetyl K15 antibody. Suitable for PepArr, WB, IHC-P, ChIP-seq and reacts with , Human, Mouse samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP955Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody only detects Histone H2B acetylated on Lysine 15.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--chip-sequencing-img175997.jpg "ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)")
ChIP-sequencing - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 μg of ab62335 [EP955Y]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.![Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--western-blot-img6802.jpg "Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)")
Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
The bottom image shows beta Tubulin as a positive control.
All lanes: Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335) at 1/2000 dilution
Lane 1: HeLa cell lysate (untreated) at 10 µg
Lane 2: HeLa cell lysate (treated with TSA) at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135392.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
![Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-acetyl-k15-antibody-ep955y-chip-grade-ab62335--peptide-array-img436302.jpg "Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)")
Peptide Array - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
ab62335 at 0.5 μg/mL was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). A Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution was used as the secondary antibody. Washing buffer and time was protein Array Washing Buffer, 2*10mins and 2*5mins and blocking buffer was 5% BSA in TBST.
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
Scanner details: Innoscan 710
Downloads
Product protocols
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