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Rabbit Polyclonal H2B acetyl K20 antibody. Suitable for ICC, IP, ChIP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC4 acetyl K20.

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Images

Immunocytochemistry - Anti-Histone H2B (acetyl K20) antibody (AB240890), expandable thumbnail
  • Western blot - Anti-Histone H2B (acetyl K20) antibody (AB240890), expandable thumbnail
  • Immunoprecipitation - Anti-Histone H2B (acetyl K20) antibody (AB240890), expandable thumbnail
  • ChIP - Anti-Histone H2B (acetyl K20) antibody (AB240890), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody (AB240890), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human H2BC4 acetyl K20. The exact immunogen used to generate this antibody is proprietary information. Database link P62807

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPChIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/2000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 5 μg per reaction.

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Rabbit Polyclonal H2B acetyl K20 antibody. Suitable for ICC, IP, ChIP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC4 acetyl K20.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human H2BC4 acetyl K20. The exact immunogen used to generate this antibody is proprietary information. Database link P62807
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry - Anti-Histone H2B (acetyl K20) antibody (ab240890), expandable thumbnail

    Immunocytochemistry - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30mM sodium butyrate for 4hr) stained for Histone H2B (acetyl K20) using ab240890 at 1/15 dilution in ICC.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight.

    The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Western blot - Anti-Histone H2B (acetyl K20) antibody (ab240890), expandable thumbnail

    Western blot - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    All lanes: Western blot - Anti-Histone H2B (acetyl K20) antibody (ab240890) at 1/100 dilution

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

    Lane 2: A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

    Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr

    Lane 4: HEK-293 whole cell lysate, untreated (-)

    Lane 5: A549 whole cell lysate, untreated (-)

    Lane 6: K562 whole cell lysate, untreated (-)

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 14 kDa

  • Immunoprecipitation - Anti-Histone H2B (acetyl K20) antibody (ab240890), expandable thumbnail

    Immunoprecipitation - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    Histone H2B (acetyl K20) was immunoprecipitated from 500 μg A549 (human lung carcinoma cell line) whole cell lysate (treated with 30mM sodium butyrate for 4hr) with ab240890 at 1/200 dilution.

    Lane 1: Rabbit control IgG IP in A549 whole cell lysate (treated with 30mM sodium butyrate for 4hr).

    Lane 2: ab240890 IP in A549 whole cell lysate (treated with 30mM sodium butyrate for 4hr).

    Lane 3: A549 whole cell lysate (treated with 30mM sodium butyrate for 4hr) 20 μg (Input).

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

    All lanes: Immunoprecipitation - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    Predicted band size: 14 kDa

  • ChIP - Anti-Histone H2B (acetyl K20) antibody (ab240890), expandable thumbnail

    ChIP - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    HeLa (human epithelial cell line from cervix adenocarcinoma; 106, treated with 30mM sodium butyrate for 4hr) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240890 or a control normal rabbit IgG.

    The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody (ab240890), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody (ab240890)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30mM sodium butyrate for 4hr) stained for Histone H2B (acetyl K20) using ab240890 at 1/50 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was an Alexa-Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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