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AB271344

Anti-Histone H2B (acetyl K20) antibody [RM235]

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Rabbit Recombinant Monoclonal H2B acetyl K20 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC1 acetyl K20.

View Alternative Names

HIST1H2BA, TSH2B, H2BC1, Histone H2B type 1-A, Testis-specific histone H2B, TSH2B.1, hTSH2B, H2BK20ac

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K20) using ab271344 at 2 μg/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green).``

ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • ELISA

Supplier Data

ELISA - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

ab271344 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)
  • WB

Supplier Data

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (AB271344)

All lanes:

Western blot - Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344) at 0.5 µg/mL

Lane 1:

Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with sodium butyrate

Lane 2:

Acid extracts of untreated HeLa cells

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM235

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ELISA, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H2BC1 acetyl K20. The exact immunogen used to generate this antibody is proprietary information.

Q96A08

Specificity

No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.2-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells (By similarity). Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones (By similarity). Core component of nucleosome (By similarity). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (By similarity). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (By similarity). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity). Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear (PubMed : 21249133).
See full target information H2BC1 acetyl K20
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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