AB222770

Anti-Histone H2B (acetyl K23) antibody [RM260]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal H2B antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acK12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.

View Alternative Names

H2BFS, H2BS1, H2BC12L, Histone H2B type F-S, H2B-clustered histone 12 like, H2B.S histone 1, Histone H2B.s, H2B/s, H2BFF, HIST1H2BB, H2BC3, Histone H2B type 1-B, H2B-clustered histone 3, Histone H2B.1, Histone H2B.f, H2B/f, H2BFR, HIST1H2BJ, H2BC11, Histone H2B type 1-J, Histone H2B.1, Histone H2B.r, H2B/r, H2BFH, H2BFN, HIST1H2BO, H2BC17, Histone H2B type 1-O, H2B-clustered histone 17, Histone H2B.2, Histone H2B.n, H2B/n, H2BFB, HIRIP2, HIST1H2BD, H2BC5, Histone H2B type 1-D, H2B-clustered histone 5, HIRA-interacting protein 2, Histone H2B.1 B, Histone H2B.b, H2B/b

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K23) (red) using ab222770 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).

Supplier Data

Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

All lanes:

Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770) at 0.5 µg/mL

Lane 1:

Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate

Lane 2:

Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated

Lane 3:

Recombinant Histone H2B

Predicted band size: 14 kDa

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ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

ELISA

Supplier Data

ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

ab222770 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in Histone H2B.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM260

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H2BC12 acK12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7. The exact immunogen used to generate this antibody is proprietary information.

O60814

Specificity

ab222770 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab222770 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

See full target information H2BC12L

Additional targets

H2BC11,H2BC17,H2BC3,

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4103 PubMed37460559

2023

Epigenetic mechanisms to propagate histone acetylation by p300/CBP.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Kikuchi,Satoshi Morita,Masatoshi Wakamori,Shin Sato,Tomomi Uchikubo-Kamo,Takehiro Suzuki,Naoshi Dohmae,Mikako Shirouzu,Takashi Umehara

Oncogene 41:1536-1549 PubMed35087237

2022

Targeting a splicing-mediated drug resistance mechanism in prostate cancer by inhibiting transcriptional regulation by PKCβ1.

Applications

Unspecified application

Species

Unspecified reactive species

James E Melnyk,Veronica Steri,Hao G Nguyen,Y Christina Hwang,John D Gordan,Byron Hann,Felix Y Feng,Kevan M Shokat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2B (acetyl K23) antibody [RM260]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (2)

Product promise