Rabbit Recombinant Monoclonal H2B acetyl K23 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
ELISA | WB | ICC/IF | |
---|---|---|---|
Human | Expected | Tested | Tested |
Synthetic peptide | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Histone H2B type 1-J, Histone H2B type 1-O, Histone H2B type 1-B, Histone H2B type 1-D, H2BS1
H2BFT, HIRIP1, HIST1H2BK, H2BC12, H2BFT, HIRIP1, HIST1H2BK, Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, H2BK23ac
Rabbit Recombinant Monoclonal H2B acetyl K23 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
RM260
Affinity purification Protein A
ab222770 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
ab222770 was purified from an animal origin–free culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K23) (red) using ab222770 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
All lanes: Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770) at 0.5 µg/mL
Lane 1: Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate
Lane 2: Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated
Lane 3: Recombinant Histone H2B
Predicted band size: 14 kDa
ab222770 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in Histone H2B.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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