Skip to main content

Rabbit Recombinant Monoclonal H2B acetyl K23 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770), expandable thumbnail
  • Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770), expandable thumbnail
  • ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (AB222770), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7. The exact immunogen used to generate this antibody is proprietary information. Database link O60814

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBICC/IF
Human
Expected
Tested
Tested
Synthetic peptide
Tested
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

0.50000-2.00000 µg/mL

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.50000-2.00000 µg/mL

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Targets

Histone H2B type 1-J, Histone H2B type 1-O, Histone H2B type 1-B, Histone H2B type 1-D, H2BS1

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H2B acetyl K23 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human H2BC12 acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7. The exact immunogen used to generate this antibody is proprietary information. Database link O60814
Clone number

RM260

Purification technique

Affinity purification Protein A

Specificity

ab222770 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.

Concentration
Loading...
Purification notes

ab222770 was purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K23) (red) using ab222770 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).

  • Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770), expandable thumbnail

    Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

    All lanes: Western blot - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770) at 0.5 µg/mL

    Lane 1: Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate

    Lane 2: Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated

    Lane 3: Recombinant Histone H2B

    Predicted band size: 14 kDa

  • ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770), expandable thumbnail

    ELISA - Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)

    ab222770 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in Histone H2B.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com