Rabbit Recombinant Monoclonal H2B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 24 publications.
Images
![Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (AB52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--flow-cytometry-intracellular-img90588.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (AB52599)")
![Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--western-blot-img56561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109569.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427924.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (AB52599)")
![Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--western-blot-img417134.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2B antibody [EP957Y] (AB52599)")
Publications
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- Frontiers in immunology 12:7796672022Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Xia,Yu Liu,Zhiwei Zhang,Yajuan Gong,Tianyi Yu,Dan Zhao,Wen Qiu,Yingwei Wang,Jing ZhangPubMed 35046941
- Molecular therapy. Nucleic acids 26:1433-14452021circRNA-DURSA regulates trophoblast apoptosis via miR-760-HIST1H2BE axis in unexplained recurrent spontaneous abortion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinyue Tang,Long Bai,Zhe Wan,Shan Wan,Yu Xiang,Yeqing Qian,Long Cui,Jiali You,Xiaoling Hu,Fan Qu,Yimin ZhuPubMed 34938599
- PLoS biology 19:e30012972021Long noncoding RNA PM maintains cerebellar synaptic integrity and Cbln1 activation via Pax6/Mll1-mediated H3K4me3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Jin,Bowen Zhang,Junxia Lu,Yingdong Song,Wei Wang,Wei Zhang,Fanghong Shao,Meng Gong,Meiting Wang,Xiaolin Liang,Shuqin Li,Zhi Zhang,Ge Shan,Xiangting WangPubMed 34111112
- Frontiers in immunology 12:6546492021Antibodies Against Alkaline Protease Directly Enhance Disruption of Neutrophil Extracellular Traps Mediated by This Enzyme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChendi Jing,Chenghua Liu,Yu Liu,Ruli Feng,Run Cao,Zhangchun Guan,Bo Xuan,Yaping Gao,Qi Wang,Nana Yang,Yuanfang Ma,Lefu Lan,Jiannan Feng,Beifen Shen,Hui Wang,Yanyan Yu,Guang YangPubMed 33868297
- Cells 10:20213D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Mi Jeong,ChulHwan Bang,MinJi Park,Sun Shin,Seokhwan Yun,Chul Min Kim,GaHee Jeong,Yeun-Jun Chung,Won-Soo Yun,Ji Hyun Lee,Songwan JinPubMed 33800001
- Cell research 31:664-6832021Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianteng Wang,Xiaolin Hu,Wanlu Song,Hui Xu,Zhengtao Xiao,Rongyao Huang,Qingran Bai,Fan Zhang,Yongzhen Chen,Yu Liu,Jianhuo Fang,Xin Li,Qin Shen,Haitao Zhao,Xuerui YangPubMed 33432115
- Experimental and therapeutic medicine 20:2639-26482020E3 ubiquitin ligase HRD1 modulates the circadian clock through regulation of BMAL1 stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongkai Guo et. al.PubMed 32765757
- Nature communications 11:11132020A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivier G de Jong et. al.PubMed 32111843
- Clinical and experimental immunology 199:1-82019Neutrophil proteases degrade autoepitopes of NET-associated proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC M de Bont,N Eerden,W C Boelens,G J M PruijnPubMed 31661552
- Journal of extracellular vesicles 8:15795412019Subtypes of tumour cell-derived small extracellular vesicles having differently externalized phosphatidylserine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSachiko Matsumura,Tamiko Minamisawa,Kanako Suga,Hiromi Kishita,Takanori Akagi,Takanori Ichiki,Yuki Ichikawa,Kiyotaka ShibaPubMed 30834072
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20000 | Notes - |
Species Rat | Dilution info 1/20000 | Notes - |
Species Mouse | Dilution info 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Alternative names
H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, HIST1H2BI, H2BC10, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, H2BFK, HIST1H2BG, HIST1H2BE, H2BFH, H2BC6, HIST1H2BC, H2BFL, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l
Recommended products
Rabbit Recombinant Monoclonal H2B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 24 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP957Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (ab52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--flow-cytometry-intracellular-img90588.jpg "Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (ab52599)")
Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (ab52599)
Overlay histogram showing HeLa cells stained with ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--western-blot-img56561.jpg "Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)")
Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)
All lanes: Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/20000 dilution
All lanes: A431 cell lysate at 10 µg
SecondaryAll lanes: HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109569.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52599 at a dilution of 1/250-1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427924.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)")
This image is courtesy of a customer review submitted by Natalie PapazianImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] (ab52599)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human bone marrow tissue staining with ab52599 at 1/200 dilution. Secondary antibody was Alexa Fluor™ 555 Donkey anti-Rabbit IgG (H+L) at 1/200 dilution. Samples were incubated with the primary antibody with PBS for 1 hour. Blocking was done using 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with 1mM EDTA PH8.0.
![Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-ab52599--western-blot-img417134.jpg "Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)")
Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Histone H2B antibody [EP957Y] (ab52599) at 1/5000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 180s
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