Rabbit Recombinant Monoclonal H2B antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--flow-cytometry-intracellular-img90588.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109569.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842)")
![Western blot - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--western-blot-img417134.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (AB239842)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Alternative names
H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, HIST1H2BI, H2BC10, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, H2BFK, HIST1H2BG, HIST1H2BE, H2BFH, H2BC6, HIST1H2BC, H2BFL, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l
Recommended products
Rabbit Recombinant Monoclonal H2B antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP957Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239842 is the carrier-free version of Anti-Histone H2B antibody [EP957Y] ab52599.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--flow-cytometry-intracellular-img90588.jpg "Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)")
Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)
Overlay histogram showing HeLa cells stained with Anti-Histone H2B antibody [EP957Y] ab52599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Histone H2B antibody [EP957Y] ab52599, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H2B antibody [EP957Y] ab52599).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109569.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Histone H2B antibody [EP957Y] ab52599 at a dilution of 1/250-1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H2B antibody [EP957Y] ab52599).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842), expandable thumbnail](https://content.abcam.com/products/images/histone-h2b-antibody-ep957y-bsa-and-azide-free-ab239842--western-blot-img417134.jpg "Western blot - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)")
Western blot - Anti-Histone H2B antibody [EP957Y] - BSA and Azide free (ab239842)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H2B antibody [EP957Y] ab52599).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.All lanes: Western blot - Anti-Histone H2B antibody [EP957Y] (Anti-Histone H2B antibody [EP957Y] ab52599) at 1/5000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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