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Human Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide samples. Cited in 1 publication.

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Images

ChIP - Anti-Histone H2B antibody [IGX4228H] (AB213288), expandable thumbnail
  • ChIP - Anti-Histone H2B antibody [IGX4228H] (AB213288), expandable thumbnail
  • Western blot - Anti-Histone H2B antibody [IGX4228H] (AB213288), expandable thumbnail
  • Peptide Array - Anti-Histone H2B antibody [IGX4228H] (AB213288), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Human

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBPepArr
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Cow
Expected
Tested
Expected
Synthetic peptide
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

2 µg chromatin for 25 µg chromatin

Notes

-

Species

Human

Dilution info

2 µg chromatin for 25 µg chromatin

Notes

-

Expected
Expected

Species

Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1 µg/mL

Notes

-

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Species

Cow

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Expected
Expected

Species

Mouse, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Human Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide samples. Cited in 1 publication.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

IGX4228H

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.
Human monoclonal antibody.

For recommended secondary antibodies -

Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) ab200622
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 647) ab200623
Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) ab201485
Rabbit Anti-Human IgG H&L (HRP) - Rabbit Anti-Human IgG H&L (HRP) ab6759

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • ChIP - Anti-Histone H2B antibody [IGX4228H] (ab213288), expandable thumbnail

    ChIP - Anti-Histone H2B antibody [IGX4228H] (ab213288)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213288 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H2B antibody [IGX4228H] (ab213288), expandable thumbnail

    ChIP - Anti-Histone H2B antibody [IGX4228H] (ab213288)

    Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213288 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H2B antibody [IGX4228H] (ab213288), expandable thumbnail

    Western blot - Anti-Histone H2B antibody [IGX4228H] (ab213288)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213288 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H2B antibody [IGX4228H] (ab213288) at 1 µg/mL

    Lane 1: CTH (Calf Thymus Histone) at 0.5 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

    Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg

    Lane 6: Histone H2B Recombinant Protein at 0.1 µg

    Lane 7: Histone H3 Recombinant Protein at 0.1 µg

    Secondary

    All lanes: HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

  • Peptide Array - Anti-Histone H2B antibody [IGX4228H] (ab213288), expandable thumbnail

    Peptide Array - Anti-Histone H2B antibody [IGX4228H] (ab213288)

    ab213288 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab213288 was 1/5000 dilution, the secondary used was Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor&trade™ Secondary Antibody at 1/50000 dilution.
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded from the Support and downloads tab.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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