Human Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide samples. Cited in 1 publication.
IgG1
Human
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ChIP | WB | PepArr | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Expected |
Cow | Expected | Tested | Expected |
Synthetic peptide | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 2 µg chromatin for 25 µg chromatin | Notes - |
Species Human | Dilution info 2 µg chromatin for 25 µg chromatin | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
H2BFT, HIRIP1, HIST1H2BK, H2BC12, H2BFT, HIRIP1, HIST1H2BK, Histone H2B type 1-K, H2B K, HIRA-interacting protein 1
Human Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide samples. Cited in 1 publication.
IgG1
Human
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IGX4228H
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
For recommended secondary antibodies -
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) ab200622
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 647) ab200623
Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) ab201485
Rabbit Anti-Human IgG H&L (HRP) - Rabbit Anti-Human IgG H&L (HRP) ab6759
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213288 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213288 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213288 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H2B antibody [IGX4228H] (ab213288) at 1 µg/mL
Lane 1: CTH (Calf Thymus Histone) at 0.5 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg
Lane 6: Histone H2B Recombinant Protein at 0.1 µg
Lane 7: Histone H3 Recombinant Protein at 0.1 µg
All lanes: HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 17 kDa
Exposure time: 30s
ab213288 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab213288 was 1/5000 dilution, the secondary used was Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor&trade™ Secondary Antibody at 1/50000 dilution.
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded from the Support and downloads tab.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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