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Rabbit Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, Dot, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide - Human samples.

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Images

ChIP - Anti-Histone H2B antibody [IGX4228R-1] (AB213225), expandable thumbnail
  • ChIP - Anti-Histone H2B antibody [IGX4228R-1] (AB213225), expandable thumbnail
  • Western blot - Anti-Histone H2B antibody [IGX4228R-1] (AB213225), expandable thumbnail
  • Peptide Array - Anti-Histone H2B antibody [IGX4228R-1] (AB213225), expandable thumbnail
  • Dot Blot - Anti-Histone H2B antibody [IGX4228R-1] (AB213225), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPWBDotPepArr
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Cow
Expected
Tested
Expected
Expected
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

2 µg chromatin for 25.00000 µg chromatin

Notes

-

Species

Human

Dilution info

2 µg chromatin for 25.00000 µg chromatin

Notes

-

Expected
Expected

Species

Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human

Dilution info

0.25 µg/mL

Notes

-

Species

Mouse

Dilution info

0.25 µg/mL

Notes

-

Species

Human

Dilution info

0.25 µg/mL

Notes

-

Species

Cow

Dilution info

0.25 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Expected
Expected

Species

Mouse, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Targets

H2BC3, H2BC12L, H2BC5, H2BC26, H2BC14, H2BC13, H2BC4, H2BC15, H2BC18, H2BC17, H2BC21 acetyl K15, H2BC9, H2BC11

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H2B acetyl K12 + K12 + K16 + K23 + K23 + K23 + K23 + K23 + K5 + K7 antibody. Suitable for ChIP, WB, Dot, PepArr and reacts with Mouse, Human, Cow, Synthetic peptide - Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

IGX4228R-1

Specificity

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

For recommended secondary antibodies -

Goat Anti-Rabbit IgG H&L (HRP) - Goat Anti-Rabbit IgG H&L (HRP) ab205718

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225), expandable thumbnail

    ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213225 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225), expandable thumbnail

    ChIP - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)


    Chromatin was prepared from NIH3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213225 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225), expandable thumbnail

    Western blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213225 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225) at 0.25 µg/mL

    Lane 1: CTH (Calf Thymus Histone) at 0.5 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

    Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 10 µg

    Lane 6: Histone H2B Recombinant Protein at 0.1 µg

    Lane 7: Histone H3 Recombinant Protein at 0.1 µg

    Secondary

    All lanes: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

    Exposure time: 1min

  • Peptide Array - Anti-Histone H2B antibody [IGX4228R-1] (ab213225), expandable thumbnail

    Peptide Array - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

    ab213225 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). The concentration of ab213225 was 1/5000 dilution, the secondary used was Goat Anti-Rabbit IgG, (H+L), Fluo 647nm conjugated at 1/50000 dilution.
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as the area under the curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including a full list of all peptides and information on the position of each peptide in the diagram, can be downloaded from the Support and downloads tab.

  • Dot Blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225), expandable thumbnail

    Dot Blot - Anti-Histone H2B antibody [IGX4228R-1] (ab213225)

    Dot blot analysis using ab213225 at 1/1000 dilution and secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.
    Lane 1: Human Histone H2B peptide
    Lane 2: Human Histone H1.4 (methyl K25) peptide
    Exposure time: 180 seconds

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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