AB242276

Anti-Histone H2B (beta-hydroxybutyryl K20) antibody

Name: Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276) | Abcam
Brand: Abcam Limited
SKU: AB242276
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal H2B beta-hydroxybutyryl K20 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H2BC15.

View Alternative Names

H2BFL, HIST1H2BC, H2BC6, H2BFH, HIST1H2BE, H2BC7, H2BFG, HIST1H2BF, H2BC8, H2BFA, HIST1H2BG, H2BC10, H2BFK, HIST1H2BI, H2BC4, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BK20bhb

2 Images

Supplier Data

Immunocytochemistry - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (AB242276)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 50mM sodium 3-hydroxybutyrate for 72 hours) labeling Histone H2B (beta-hydroxybutyryl K20) with ab242276 at 1/40 dilution in ICC.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supplier Data

Western blot - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (AB242276)

All lanes:

Western blot - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (ab242276) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 50mM Sodium 3-hydroxybutyrate for 72 hours

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 50mM Sodium 3-hydroxybutyrate for 72 hours

Lane 3:

HEK-293 whole cell lysate, untreated (-)

Lane 4:

A549 whole cell lysate, untreated (-)

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human H2BC15. The exact immunogen used to generate this antibody is proprietary information.

Q99877

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/1000", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

See full target information H2BC4 beta-hydroxybutyryl K20

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7077 PubMed39152118

2024

ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4 macrophage infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Caihua Zhang,Kang Li,Hongzhang Zhu,Maosheng Cheng,Shuang Chen,Rongsong Ling,Cheng Wang,Demeng Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2B (beta-hydroxybutyryl K20) antibody

Immunocytochemistry - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (AB242276)

Western blot - Anti-Histone H2B (beta-hydroxybutyryl K20) antibody (AB242276)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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