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Rabbit Polyclonal H2B butyryl K5 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC4 butyryl K5.

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Images

Western blot - Anti-Histone H2B (butyryl K5) antibody (AB235429), expandable thumbnail
  • Immunoprecipitation - Anti-Histone H2B (butyryl K5) antibody (AB235429), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (butyryl K5) antibody (AB235429), expandable thumbnail
  • ChIP - Anti-Histone H2B (butyryl K5) antibody (AB235429), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human H2BC4 butyryl K5. The exact immunogen used to generate this antibody is proprietary information. Database link P62807

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/2000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/cells for 6.00000 µg/cells

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10.00000 - 1/100.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Rabbit Polyclonal H2B butyryl K5 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human H2BC4 butyryl K5.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human H2BC4 butyryl K5. The exact immunogen used to generate this antibody is proprietary information. Database link P62807
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-Histone H2B (butyryl K5) antibody (ab235429), expandable thumbnail

    Western blot - Anti-Histone H2B (butyryl K5) antibody (ab235429)

    All lanes: Western blot - Anti-Histone H2B (butyryl K5) antibody (ab235429) at 1/100 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

    Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

    Lane 3: A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

    Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

    Lane 5: HeLa whole cell lysate, untreated

    Lane 6: HEK-293 whole cell lysate, untreated

    Lane 7: A549 whole cell lysate, untreated

    Lane 8: HepG2 whole cell lysate, untreated

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 14 kDa

  • Immunoprecipitation - Anti-Histone H2B (butyryl K5) antibody (ab235429), expandable thumbnail

    Immunoprecipitation - Anti-Histone H2B (butyryl K5) antibody (ab235429)

    Histone H2B (butyryl K5) was immunoprecipitated from HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate using 5 μg of ab235429.

    Lane 1: Rabbit control IgG in HEK-293 whole cell lysate.

    Lane 2: ab235429 IP in HEK-293 whole cell lysate.

    Lane 3: HEK-293 whole cell lysate (20 μg input).

    For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

    All lanes: Immunoprecipitation - Anti-Histone H2B (butyryl K5) antibody (ab235429)

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (butyryl K5) antibody (ab235429), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (butyryl K5) antibody (ab235429)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H2B (butyryl K5) with ab235429 at 1/15 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

  • ChIP - Anti-Histone H2B (butyryl K5) antibody (ab235429), expandable thumbnail

    ChIP - Anti-Histone H2B (butyryl K5) antibody (ab235429)

    HeLa (human epithelial cell line from cervix adenocarcinoma) (106, treated with 30 mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab235429 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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Product protocols

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