AB222762

Anti-Histone H2B (phospho S14) antibody [RM238]

Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal H2B phospho S14 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

H2BFC, HIST1H2BL, H2BC13, Histone H2B type 1-L, Histone H2B.c, H2B/c, H2BS14p

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2B (phospho S14) (red) using ab222762 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green). DAPI nuclear stain (blue).``

Supplier Data

Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762)

All lanes:

Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762) at 0.5 µg/mL

Lane 1:

Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole

Lane 2:

Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM238

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Specificity

ab222762 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab222762 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H2BC13 phospho S14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1079400 PubMed36865910

2023

Kaixin-San improves Aβ-induced synaptic plasticity inhibition by affecting the expression of regulation proteins associated with postsynaptic AMPAR expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Meng-Lu Wang,Shu-Ming Huang,Yu Cui,Yan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H2B (phospho S14) antibody [RM238]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762)

Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise