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Rabbit Recombinant Monoclonal H2B phospho S14 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762), expandable thumbnail
  • Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (AB222762), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

0.50000-2.00000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal H2B phospho S14 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Clone number

RM238

Purification technique

Affinity purification Protein A

Specificity

ab222762 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

Concentration
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Purification notes

ab222762 was purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2B (phospho S14) (red) using ab222762 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green). DAPI nuclear stain (blue).

  • Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762), expandable thumbnail

    Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

    All lanes: Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762) at 0.5 µg/mL

    Lane 1: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole

    Lane 2: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole

    Predicted band size: 14 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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