Rabbit Recombinant Monoclonal H2B phospho S14 antibody. Suitable for WB, ICC/IF and reacts with Human samples.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
H2BFC, HIST1H2BL, HIST1H2BL, H2BFC, H2BC13, Histone H2B type 1-L, Histone H2B.c, H2B/c, H2BS14p
Rabbit Recombinant Monoclonal H2B phospho S14 antibody. Suitable for WB, ICC/IF and reacts with Human samples.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
RM238
Affinity purification Protein A
ab222762 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
ab222762 was purified from an animal origin–free culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2B (phospho S14) (red) using ab222762 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green). DAPI nuclear stain (blue).
All lanes: Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762) at 0.5 µg/mL
Lane 1: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
Lane 2: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole
Predicted band size: 14 kDa
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